Scientific Data

Immunohistochemistry

CLEC4F/CLECSF13 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

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CLEC4F/CLECSF13 in Mouse Liver. CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse CLEC4F/CLECSF13 Monoclonal Antibody (Catalog # MAB2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry/ Immunofluorescence

Detection of Mouse CLEC4F/CLECSF13 by Immunocytochemistry/Immunofluorescence

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Detection of Mouse CLEC4F/CLECSF13 by Immunocytochemistry/Immunofluorescence CETP protein is not co‐localized with Ly6C protein, but does co‐localize with Clec4f protein. Livers of non‐injected female APOE*3‐Leiden‐CETP mice were assayed for co‐localization of CETP and F4/80 (A), Ly6C (B) and Clec4f (C). Red; F4/80, Ly6C or Clec4f, Green; CETP, Blue; DAPI. Double headed arrows indicate co‐localization, single‐headed arrows indicate no co‐localization. CETP indicates cholesteryl ester transfer protein; Clec4f, C‐type lectin domain family 4; Ly6C, lymphocyte antigen 6 complex locus C. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29525783), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse CLEC4F/CLECSF13 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CLECSF4F, rhCLEC4D, rhCLEC4E, recombinant mouse (rm) OCIL, and rmOCIL-rp2 is observed.

Source

Monoclonal Rat IgG_2A Clone # 370901

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13
Ala65-Gly548
Accession # P70194

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CLEC4F/CLECSF13 (Catalog # 2784-CL)

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

mCLEC4F MAb (Cl 3709 (25 ug)