Acetyl-CoA Carboxylase (C83B10) Rabbit mAb
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Acetyl-CoA Carboxylase (C83B10) Rabbit mAb

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品牌: CST
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    分子量:
    280
    280
    反应种属:
    Human,Mouse,Rat,Hamster,
    Human,Mouse,Rat,Hamster,
    展开
    产品介绍
    产品信息
    抗原名称
    CoA Carboxylase
    来源纯化
    使用与人乙酰辅酶 A 羧化酶 α1 Ser523 周围的残基相对应的合成肽对动物进行免疫接种来产生单克隆抗体。
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying acetyl-CoA carboxylase 1/acetyl-CoA carboxylase 2. Cited in 650 publications. Validated for WB, WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Acetyl-CoA Carboxylase (C83B10) Rabbit Monoclonal Antibody (CST #3676) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:1000
    Simple Western™1:10 - 1:50
    Immunoprecipitation1:100
    Immunohistochemistry (Paraffin)1:50 - 1:200
    Immunofluorescence (Immunocytochemistry)1:100 - 1:200
    Flow Cytometry (Fixed/Permeabilized)1:200 - 1:800
    分子量
    280
    研究领域
    癌症,代谢,神经科学
    应用
    反应种属
    Human,Mouse,Rat,Hamster,
    目标/特异性

    Specificity/Sensitivity

    Acetyl-CoA Carboxylase (C83B10) Rabbit mAb 可检测乙酰辅酶 A 羧化酶蛋白的所有同工型的内源水平。

    物种反应性:

    人, 小鼠, 大鼠, 仓鼠

    敏感性
    Endogenous
    背景
    背景
    乙酰辅酶 A 羧化酶 (ACC) 可催化乙酰辅酶 A 羧化形成丙二酰辅酶 A (1)。它是脂肪酸生物合成和氧化过程中的一种关键酶 (1)。在啮齿动物中,265 kDa ACC1 (ACCα) 形式主要在脂肪生成组织中表达,而 280 kDa ACC2 (ACCβ) 则是氧化组织中的主要同工型 (1,2)。但在人类中,ACC2 是脂肪生成和氧化组织中的主要同工型 (1,2)。AMPK 在 Ser79 位点的磷酸化或 PKA 在 Ser1200 位点的磷酸化会抑制 ACC 的酶活性 (3)。ACC 是抗肥胖药物的潜在靶标 (4,5)。 1.Castle, J.C. et al. (2009) PLoS One 4, e4369. 2.Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86. 3.Ha, J. et al. (1994) J Biol Chem 269, 22162-8. 4.Abu-Elheiga, L. et al. (2001) Science 291, 2613-6. 5.Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50. 6.Fullerton, M.D. et al. (2013) Nat Med 19, 1649-54.
    研究领域
    癌症,代谢,神经科学
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #52923.
    数据库链接
    Entrez-Gene ID
    31,32
    UniProt ID
    Q13085,O00763

    参考图片

    Flow cytometric analysis of 293 cells, using Acetyl-CoA Carboxylase (C83B10) Rb mAb (blue) compared to a nonspecific negative control antibody (red).

    流式细胞检测293细胞,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗(蓝), 对照为非特异性阴性抗体对照(红)

    Western blot analysis of cell extracts from various cell lines, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

    Wetern blot检测多种细胞提取物,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

    Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (red). Blue pseudocolor=Draq5™ (fluorescent DNA dye).

    激光共聚焦免疫荧光检测NIH/3T3细胞,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗(红),蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084) 。

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

    免疫组织化学检测石蜡包埋的人乳腺癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

    免疫组织化学检测石蜡包埋的人结肠癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma, using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

    免疫组织化学检测石蜡包埋的人肝脏细胞癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb.

    免疫组织化学检测石蜡包埋的人肺癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗。

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb in the presence of control peptide (left) or Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062 (right).

    免疫组织化学检测石蜡包埋的人乳腺癌组织,所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗,左为对照肽,右为 Acetyl-CoA Carboxylase (C83B10) Blocking Peptide #1062

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    货号:
    3676T
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