Creatinine Assay Kit (1 KIT)
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Creatinine Assay Kit (1 KIT)

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品牌: R&D Systems
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    Product Summary

    The Parameter Creatinine assay is a 30 minute chemical analysis designed to measure Creatinine in urine.

    Recovery

    The recovery of Creatinine spiked to levels throughout the range of the assay was evaluated.

    Sample TypeAverage % RecoveryRange %
    Urine (n=4)9692-97

    Linearity

    To assess the linearity, nine urine samples were serially diluted with deionized or distilled water and assayed in two lots of kit reagents.
    Multi-species Creatinine ELISA Linearity

    Scientific Data

    Multi-species Creatinine ELISA Standard Curve

    Assay Procedure

    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Standard, Control, or Sample
    4.   Add 50 µL of Standard, control, or sample to each well.

    5. 100 µL Alkaline Picrate Solution
    6.   Add 100 µL of Alkaline Picrate Solution to each well.
    7.   Incubate at room temperature for 25-35 minutes. Read at 490 nm.

    Creatinine Parameter Assay Kit Summary

    Format
    96-well strip plate
    Assay Length
    < 1 hour
    Sample Type & Volume Required Per Well
    Urine (10 uL)
    Sensitivity
    0.07 mg/dL
    Assay Range
    0.3 - 20 mg/dL (Urine)
    Specificity
    Measures Creatinine levels in urine
    Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
    Interference
    Interference observed with other substances.
    背景
    别名
    Creatinine
    背景

    Background: Creatinine

    Creatinine is a non-enzymatic metabolite of phospho-Creatine, a molecule used primarily by muscle tissue as a store for high-energy phosphate that can be utilized for the production of ATP. Creatinine diffuses into the blood and is excreted by the kidneys at a relatively constant rate. Creatinine levels in the urine are used as a measurement of renal function.

    Alternate Names:
    Creatinine
    研究领域
    制备和贮存
    保存方式

    Preparation and Storage

    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Storage
    Storage conditions vary by component. See product insert for storage instructions.
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    货号:
    KGE005
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    询价
    1KIT
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