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hGM-CSF MAb (Cl 3209 (500 ug)

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hGM-CSF MAb (Cl 3209 (500 ug)

品牌： R&D Systems

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来源宿主：

Mouse IgG1

实验应用：

Western Blot(1 µg/mL)
Immunocytochemistry(8-25 µg/mL)

产品介绍

产品信息

耦联标记

Unconjugated

纯化方式

Protein A or G purified from hybridoma culture supernatant

抗原名称

colony stimulating factor 2 (granulocyte-macrophage)， Colony-stimulating factor， CSF， CSF2， CSF-2， G

宿主

Mouse IgG1

免疫原

E. coli-derived recombinant human GM-CSF
Ala18-Glu144
Accession # P04141

克隆号

3209

Scientific Data

Immunocytochemistry

View Larger

GMCSF in Human PBMCs. Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization

View Larger

Cell Proliferation Induced by GM‑CSF and Neutralization by Human GM‑CSF Antibody. Recombinant Human GM-CSF (Catalog # 215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM-CSF Monoclonal Antibody (Catalog # MAB215). The ND₅₀ is typically 0.075-0.45 µg/mL.

Human GM-CSF Antibody Summary

Species Reactivity

Human

Specificity

Detects human GM-CSF in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 3209

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GM-CSF
Ala18-Glu144
Accession # P04141

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human GM-CSF (Catalog # 215-GM)
under non-reducing conditions only

Immunocytochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize GM‑CSF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.075-0.45 µg/mL in the presence of 0.1 ng/mL Recombinant Human GM‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用

实验应用

Western Blot(1 µg/mL)
Immunocytochemistry(8-25 µg/mL)

背景

别名

colony stimulating factor 2 (granulocyte-macrophage),Colony-stimulating factor,CSF,CSF2,CSF-2,GMCSF,GM-CSF,GMCSFgranulocyte-macrophage colony-stimulating factor,granulocyte-macrophage colony stimulating factor,MGC131935,MGC138897,Molgramostim,molgramostin,Sargramostim,Granulocyte Macrophage Growth Factor

背景

Background: GM-CSF

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Long Name

Granulocyte Macrophage Growth Factor

Entrez Gene IDs

1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)

Alternate Names

colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim

研究领域

Related Research Areas

制备和贮存

溶解方法

Reconstitute at 0.5 mg/mL in sterile PBS.

保存方式

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Cell Proliferation Induced by GM‑CSF and Neutralization by Human GM‑CSF Antibody. Recombinant Human GM‑CSF (Catalog # 215-GM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Human GM‑CSF Monoclonal Antibody (Catalog # MAB215). The ND₅₀ is typically 0.3-0.5 µg/mL.

GMCSF in Human PBMCs. Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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货号：

MAB215-500

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