Product Details

The UC10-4F10-11 monoclonal antibody reacts with mouse CTLA-4 (cytotoxic T lymphocyte antigen-4) also known as CD152. CTLA-4 is a 33 kDa cell surface receptor encoded by the Ctla4 gene that belongs to the CD28 family of the Ig superfamily. CTLA-4 is expressed on activated T and B lymphocytes. CTLA-4 is structurally similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to the B7 family members B7-1 (CD80) and B7-2 (CD86). Upon ligand binding, CTLA-4 negatively regulates cell-mediated immune responses. CTLA-4 plays roles in induction and/or maintenance of immunological tolerance, thymocyte development, and regulation of protective immunity. The critical role of CTLA-4 in immune down-regulation has been demonstrated in CTLA-4 deficient mice, which succumb at 3-5 weeks of age due to the development of a lymphoproliferative disease. CTLA-4 is among a group of inhibitory receptors being explored as cancer treatment targets through immune checkpoint blockade. The UC10-4F10-11 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, allowing for CD28 binding.

Specifications

Isotype	Armenian hamster IgG
Recommended Isotype Control(s)	InVivoPlus polyclonal Armenian hamster IgG
Recommended Dilution Buffer	InVivoPure pH 6.5 Dilution Buffer
Conjugation	This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Immunogen	Mouse CTLA-4 IgG2a fusion protein
Reported Applications	in vivo CTLA-4 neutralization in vitro CTLA-4 neutralization Flow cytometry Western blot
Formulation	PBS, pH 6.5 Contains no stabilizers or preservatives
Endotoxin*	<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation*	<5% Determined by SEC
Purity	>95% Determined by SDS-PAGE
Sterility	0.2 μM filtered
Production	Purified from cell culture supernatant in an animal-free facility
Purification	Protein G
RRID	AB_1107598
Molecular Weight	150 kDa
Murine Pathogen Tests*	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

* Additional quality control measures for our InVivoPlus™ products include advanced binding validation, murine pathogen screening, protein aggregation screening, and ultra-low endotoxin levels. The superior quality of our InVivoPlus™ products will meet and exceed the strict demands and rigorous standards required for in vivo research. Learn more about the InVivoPlus™ difference here.

组成成分

IGG-免疫球蛋白G型抗体

应用

反应种属

mouse

背景

研究领域

Cancer Biology, Immune Checkpoint Protein, Immunology

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货号：

BP0032-5MG

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