Cy5-conjugated AffiniPure™ Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot)

Cy5-conjugated AffiniPure™ Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot)

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品牌: Jackson
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    反应种属:
    Mouse
    Mouse
    来源宿主:
    山羊
    山羊
    产品介绍
    产品信息
    抗原名称
    IgG (H+L)
    宿主
    山羊

    Target: Mouse
    Host: Goat
    Antibody Format: Whole IgG
    Specificity: IgG (H+L)
    Minimal Cross Reactivity: Human, Bovine, Horse, Rabbit, Swine Serum Proteins
    Conjugate: Cyanine Cy™5
    Product Category: Cyanine Conjugates for Permanent Mounting
    Clonality: Polyclonal
    RRID: AB_2338713

    Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, horse, rabbit and swine serum proteins, but it may cross-react with immunoglobulins from other species.

    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

    Physical State: Freeze-dried solid

    Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
    Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
    Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


    Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
    Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
    Preservative: 0.05% Sodium Azide

    Suggested Working Concentration or Dilution Range:

    1:100 - 1:400 for most applications

    Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

    Cyanine Cy™5

    Amax: 650 Emax: 670nm

    Cy5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm. They can be excited to about 98% of maximum with a krypton/argon laser (647 nm line) or to about 63% of maximum with a helium/neon laser (633 nm line). Cy5 can be used with a variety of other fluorophores for multiple labeling due to a wide separation of its emission from that of shorter wavelength-emitting fluorophores. However, we recommend Alexa Fluor® 647 as the preferred far-red-fluorescing dye because it is brighter than Cy5 in aqueous mounting media. The reverse is true when mounting in non-polar, plastic mounting media, i.e. Cy5 is brighter than other far-red-fluorescing dyes, including Alexa Fluor® 647 and DyLight 650.

    组成成分
    荧光染料山羊抗小鼠免疫球蛋白G抗体;缓冲液(0.01M磷酸钠、0.25M氯化钠);稳定剂(1.5%牛血清白蛋白);防腐剂(0.05%叠氮化钠溶液)
    应用
    反应种属
    Mouse
    交叉反应性
    Human, Bovine, Horse, Rabbit, Swine Serum Proteins
    最小交叉反应
    Human, Bovine, Horse, Rabbit, Swine Serum Proteins
    目标/特异性
    IgG (H+L)
    数据库链接
    研究资源识别码
    AB_2338713
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    货号:
    115-175-146
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    询价
    1.5mg
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