Mouse Dendritic Cell Enrichment Set - DM

Magnetic Bead

The BD IMag™ Mouse Dendritic Cell Enrichment Set - DM is used for the negative selection of dendritic cells (DC) from mouse spleen or lymph node. The Biotinylated Mouse Dendritic Cell Enrichment Cocktail contains monoclonal antibodies that recognize antigens expressed on peripheral erythrocytes and leukocytes that are not DC. The BD IMag Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. With these two components, the BD IMag Mouse Dendritic Cell Enrichment Set - DM avoids the inadvertent activation of the enriched DC by using reagents that do not directly bind to those DC. This Set has been optimized for use with the BD IMag™ Cell Separation Magnet, and it contains sufficient reagents to label 10^9 leukocytes.   The Dendritic Cell Enrichment Cocktail component is comprised of the following biotin-conjugated monoclonal antibodies: Anti-mouse CD2 (LFA-2), clone RM2-5 Anti-mouse CD3e (CD3 ε chain), clone 145-2C11 Anti-mouse CD45R/B220, clone RA3-6B2 Anti-mouse CD49b (Integrin α2 chain), clone HMα2 Anti-mouse CD147 (Basigin), clone RL73 Anti-mouse Ly-6g and Ly-6C (Gr-1), clone RB6-8C5 Anti-mouse TER-119/Erythroid Cells, clone TER-119

Rat IgG2a, κ

Cell separation (Routinely Tested)