BD IMag™ Human Dendritic Cell Enrichment Set - DM

Enrichment of dendritic cells from human blood. PBMC were labeled with the BD IMag™ Human dendritic cell Enrichment Set - DM (Cat. No. 558420) and separated on the BD IMag™ Cell Separation Magnet(Cat. No. 552311) according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with a lineage cocktail consisting of APC Mouse Anti-Human CD3 (Cat. No. 555335), CD19 (Cat. No. 555415), CD14 (Cat. No. 555399), and CD56 (Cat. No. 555518) and a dendritic cell cocktail consisting of PE Mouse Anti-Human CD11c (Cat. No. 555392), CD123 (Cat. No. 555644), CD16 (Cat. No. 555407) and CD34 (Cat. No. 550761). Dead cells were excluded by staining with 7-Amino-actinomycin D (7-AAD) (Cat. No. 559925). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of dendritic cells is indicated in the upper-left corner of each panel. Panel A shows unseparated PBMC. Panel B shows the twice-enriched fraction after three 6-minute magnetic separations with an additional 10-minute separation. Panel C shows the positive fraction.

Enrichment of dendritic cells from human blood. PBMC were labeled with the BD IMag™ Human dendritic cell Enrichment Set - DM (Cat. No. 558420) and separated on the BD IMag™ Cell Separation Magnet(Cat. No. 552311) according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with a lineage cocktail consisting of APC Mouse Anti-Human CD3 (Cat. No. 555335), CD19 (Cat. No. 555415), CD14 (Cat. No. 555399), and CD56 (Cat. No. 555518) and a dendritic cell cocktail consisting of PE Mouse Anti-Human CD11c (Cat. No. 555392), CD123 (Cat. No. 555644), CD16 (Cat. No. 555407) and CD34 (Cat. No. 550761). Dead cells were excluded by staining with 7-Amino-actinomycin D (7-AAD) (Cat. No. 559925). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of dendritic cells is indicated in the upper-left corner of each panel. Panel A shows unseparated PBMC. Panel B shows the twice-enriched fraction after three 6-minute magnetic separations with an additional 10-minute separation. Panel C shows the positive fraction.