hDkk-1 MAb (Cl 14111 (25 ug)

hDkk-1 MAb (Cl 14111 (25 ug)

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品牌: R&D Systems
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    来源宿主:
    Mouse IgG1
    Mouse IgG1
    实验应用:
    Western Blot
    Western Blot
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A or G purified from hybridoma culture supernatant
    抗原名称
    dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1
    宿主
    Mouse IgG1
    免疫原
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
    Thr32-His266
    Accession # O94907
    克隆号
    141119

    Human Dkk-1 Antibody Summary

    Species Reactivity
    Human
    Specificity
    Detects human Dkk-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross‑reactivity with recombinant mouse (rm) Dkk-1 and no cross-reactivity with rmDkk-2, rhDkk-3, or rhDkk-4.
    Source
    Monoclonal Mouse IgG1 Clone # 141119
    Purification
    Protein A or G purified from hybridoma culture supernatant
    Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
    Thr32-His266
    Accession # O94907
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

    Applications

    Recommended Concentration
    Sample
    Western Blot
    1 µg/mL
    Recombinant Human Dkk‑1 (Catalog # 5439-DK)
    under non-reducing conditions only

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Western Blot
    背景
    别名
    dickkopf (Xenopus laevis) homolog 1,dickkopf homolog 1 (Xenopus laevis),dickkopf related protein-1,Dickkopf-1,dickkopf-related protein 1,Dkk1,Dkk-1,hDkk-1,SKdickkopf-1 like,Dickkopf-1
    背景

    Background: Dkk-1

    Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development.

    Long Name
    Dickkopf-1
    Entrez Gene IDs
    22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)
    Alternate Names
    dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
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    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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    货号:
    MAB1096-SP
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    询价
    25ug
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    500ug
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