DNase Ⅰ

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DNase Ⅰ

品牌： UA BIOSCIENCE

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参数

分子量：

72kDa (Reducing)

纯度：

>95% by SDS-PAGE&HPLC

产品介绍

产品信息

耦联标记

Unconjugated

分子量

72kDa (Reducing)

生物活性

2U/ul

酶活定义

One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer.

纯度

>95% by SDS-PAGE&HPLC

性状

Liquid

表达系统

E.coli

缓冲体系

10 mM Tris-HCl, 2 mM CaCl2 ,50% Glycerol，(pH 7.6, 25°C)

组合货号

Storage Solution: 2 U/ul DnaseⅠ、10mM Tris-Hcl、2mM CaCl2、50%Glycerol (pH7.6, 25℃)

10*Reaction Buffer: 100mM Tris-Hcl、25mM MgCl2、5mM CaCl2 (pH7.6, 25℃)

标签

His Tag,MBP Tag

种属

Bovine Pancreatic

电泳JSON

[{"imgUrl":"https://univ-pmc.oss-cn-shanghai.aliyuncs.com/product.imager.attribute/0/b0fc9134-ed0d-4f24-9da1-c4142ebe1dc9.png","description":"

1μg (R: reducing condition, N: non-reducing\ncondition).

"}]

背景

别名

DNASE,Deoxyribonuclease-1

背景

DNase I (Deoxyribonuclease I), can digest single or double-stranded DNA to produce mono deoxynucleotides or single or double-stranded oligo deoxynucleotides, its optimal working pH range is 7-8. DNase I activity is dependent on Ca2+ and can be activated by other bivalent metal ions such as Mg2+, Mn2+, Zn2+, etc. In the presence of Mg2+, the enzyme can randomly recognize and cut any site on any strand of DNA. In the presence of Mn2+, two strands of DNA can be cut at the same site to form sticky ends with flat ends or 1-2 nucleotides protruding.

制备和贮存

保存方式

Store at -25 ~ -15℃ for 2 years

文献

[1] Vanecko S, Laskowski M. Studies of the Specificity of Deoxyribonuclease I[J]. Journal of Biological Chemistry, 1961, 236(236):3312-6.

[2] Kienzle N, Young D, Zehntner S, et al. DNaseI treatment is a prerequisite for the amplification of cDNA from episomal-based genes[J]. Biotechniques, 1996, 20(4):612-6.

[3] Michael,R, Green, et al. Human β-globin pre-mRNA synthesized in vitro is accurately spliced in xenopus oocyte nuclei[J].Cell, 1983, 32(3):681-694.

参考图片

1μg (R: reducing condition, N: non-reducing condition).

The results of 1μg pBR322 plasmid digestion separated under different quantity of DnaseⅠ, The reaction was incubated for 10 minutes at 37°C, and 1% agarose gel was used for electrophoresis analysis after reaction.

M, marker;

Lane 1 1μg pBR322;

Lane 2 1μg pBR322 add 4U DNase I

Lane 3 1μg pBR322 add 2U DNase I

Lane 4 1μg pBR322 add 1U DNase I

Lane 5 1μg pBR322 add 0.5U DNase I

Lane 6 1μg pBR322 add 0.25U DNase I

Lane 7 1μg pBR322 add 0.125U DNase I

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货号：

UA070036-1KU

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