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SQSTM1/p62 (D6M5X) Rabbit mAb

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SQSTM1/p62 (D6M5X) Rabbit mAb

品牌： CST

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参数

分子量：

反应种属：

Mouse,Rat,

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产品介绍

产品信息

抗原名称

SQSTM1p62

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly300 of mouse SQSTM1/p62 protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying SQSTM1 mouse. Cited in 217 publications. Validated for WB, IP, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SQSTM1/p62 (D6M5X) Rabbit Monoclonal Antibody (CST #23214) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:200
Immunohistochemistry (Paraffin)	1:125 - 1:500
Immunofluorescence (Immunocytochemistry)	1:400 - 1:1600

分子量

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

应用

反应种属

Mouse,Rat,

目标/特异性

Specificity/Sensitivity

SQSTM1/p62 (D6M5X) Rabbit mAb recognizes endogenous levels of total rodent SQSTM1/p62 protein.

Species Reactivity:

Mouse, Rat

敏感性

Endogenous

背景

Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5) and independently found to interact with PKCζ (6,7). SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. 1.Kirkin, V. et al. (2009) Mol Cell 34, 259-69. 2.Seibenhener, M.L. et al. (2007) FEBS Lett 581, 175-9. 3.Komatsu, M. et al. (2010) Nat Cell Biol 12, 213-23. 4.Bjørkøy, G. et al. (2006) Autophagy 2, 138-9. 5.Joung, I. et al. (1996) Proc Natl Acad Sci USA 93, 5991-5. 6.Sanchez, P. et al. (1998) Mol Cell Biol 18, 3069-80. 7.Puls, A. et al. (1997) Proc Natl Acad Sci USA 94, 6191-6. 8.Vadlamudi, R.K. et al. (1996) J Biol Chem 271, 20235-7. 9.Wooten, M.W. et al. (2005) J Biol Chem 280, 35625-9. 10.Bjørkøy, G. et al. (2005) J Cell Biol 171, 603-14. 11.Komatsu, M. et al. (2007) Cell 131, 1149-63. 12.Pankiv, S. et al. (2007) J Biol Chem 282, 24131-45.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

18412

UniProt ID

Q64337

参考图片

Immunohistochemical analysis of paraffin-embedded mouse spleen using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

Western blot analysis of extracts from MEFs from wild-type or SQSTM1/p62 knockout mice using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). MEF SQSTM1/p62 KO cells were kindly provided by Dr. Junying Yuan, Harvard Medical School, Boston MA.

Western blot analysis of extracts from C2C12 cells, untreated (-) or treated with Chloroquine #14774 (50 μM, overnight) using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from various cell lines using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

Western blot analysis of extracts from MEFs, untreated (-) or treated with Earles Basic Salt Solution (EBSS; 4 hr; +) using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of SQSTM1 from L-929 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900, and lane 3 is SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific). Western blot was performed using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific). Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.

Immunohistochemical analysis of paraffin-embedded MEF wild-type cell pellet (left, positive) or MEF SQSTM1/p62 KO cell pellet (right, negative) using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific). MEF SQSTM1/p62 KO cells were kindly provided by Dr. Junying Yuan, Harvard Medical School, Boston MA.

Immunohistochemical analysis of paraffin-embedded mouse forestomach using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

Immunohistochemical analysis of paraffin-embedded mouse kidney using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

Immunohistochemical analysis of paraffin-embedded rat spleen using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

Immunohistochemical analysis of paraffin-embedded mouse small intestine using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

Confocal immunofluorescent analysis of wild-type MEFs, either untreated (left) or treated with Chloroquine #14774 (50 μM, 18 hours; center), and SQSTM1/p62 knock-out MEFs treated with chloroquine (right), using SQSTM1 (D6M5X) Rabbit mAb (green). Actin filaments were labeled with β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye). MEF SQSTM1/p62 KO cells were kindly provided by Dr. Junying Yuan, Harvard Medical School, Boston MA.

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货号：

23214S

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