首页 抗体 一抗 一抗
SQSTM1/p62 (D5L7G) Mouse mAb
SQSTM1/p62 (D5L7G) Mouse mAb
1/5

SQSTM1/p62 (D5L7G) Mouse mAb

分享
品牌： CST
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    分子量：
    62
    62
    反应种属：
    Human
    Human
    展开
    产品介绍
    产品信息
    抗原名称
    SQSTM1p62
    来源纯化
    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro220 of human SQSTM1 protein.
    宿主
    Mouse
    简单描述
    Monoclonal Antibody for studying SQSTM1. Cited in 177 publications. Validated for WB, IP, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SQSTM1/p62 (D5L7G) Mouse Monoclonal Antibody (CST #88588) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:1000
    Immunoprecipitation1:200
    Immunohistochemistry (Paraffin)1:400 - 1:1600
    Immunofluorescence (Immunocytochemistry)1:200 - 1:800
    分子量
    62
    研究领域
    癌症,细胞生物学,纤维化,代谢,神经科学
    应用
    反应种属
    Human
    目标/特异性

    Specificity/Sensitivity

    SQSTM1/p62 (D5L7G) Mouse mAb recognizes endogenous levels of total SQSTM1/p62 protein.

    Species Reactivity:

    Human

    敏感性
    Endogenous
    背景
    背景
    Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5) and independently found to interact with PKCζ (6,7). SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. 1.Kirkin, V. et al. (2009) Mol Cell 34, 259-69. 2.Seibenhener, M.L. et al. (2007) FEBS Lett 581, 175-9. 3.Komatsu, M. et al. (2010) Nat Cell Biol 12, 213-23. 4.Bjørkøy, G. et al. (2006) Autophagy 2, 138-9. 5.Joung, I. et al. (1996) Proc Natl Acad Sci USA 93, 5991-5. 6.Sanchez, P. et al. (1998) Mol Cell Biol 18, 3069-80. 7.Puls, A. et al. (1997) Proc Natl Acad Sci USA 94, 6191-6. 8.Vadlamudi, R.K. et al. (1996) J Biol Chem 271, 20235-7. 9.Wooten, M.W. et al. (2005) J Biol Chem 280, 35625-9. 10.Bjørkøy, G. et al. (2005) J Cell Biol 171, 603-14. 11.Komatsu, M. et al. (2007) Cell 131, 1149-63. 12.Pankiv, S. et al. (2007) J Biol Chem 282, 24131-45.
    研究领域
    癌症,细胞生物学,纤维化,代谢,神经科学
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
    数据库链接
    Entrez-Gene ID
    8878
    UniProt ID
    Q13501

    参考图片

    Western blot analysis of extracts from various cell lines using SQSTM1/p62 (D5L7G) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

    Western blot analysis of extract from HCT 116 cells, untreated (-) or starved with Earles Basic Salt Solution (EBSS; 4 hr; +) using SQSTM1/p62 (D5L7G) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

    Western blot anlaysis of extracts from HeLa cells, untreated (-) or treated with Chloroquine #14774 (50 μM, overnight; +) using SQSTM1/p62 (D5L7G) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

    Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® SQSTM1/p62 siRNA I #6394 (+), or SignalSilence® SQSTM1/p62 siRNA II #6399 (+), using SQSTM1/p62 (D5L7G) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

    Immunoprecipitation of SQSTM1/p62 protein from PANC-1 cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is SQSTM1/p62 (D5L7G) Mouse mAb. Western blot was performed using SQSTM1/p62 (D5L7G) Mouse mAb. A light chain-specific secondary antibody was used to avoid reactivity with heavy chain IgG.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SQSTM1/p62 (D5L7G) Mouse mAb.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SQSTM1/p62 (D5L7G) Mouse mAb.

    Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using SQSTM1/p62 (D5L7G) Mouse mAb.

    Immunohistochemical analysis of paraffin-embedded HDLM-2 (left) and Daudi (right) cell pellets using SQSTM1/p62 (D5L7G) Mouse mAb.

    Immunohistochemical analysis of paraffin-embedded human inflamed gall bladder using SQSTM1/p62 (D5L7G) Mouse mAb in the presence of control peptide (left) or antigen-specific peptide (right).

    Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with chloroquine (50 μM overnight; right), using SQSTM1/p62 (D5L7G) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    88588S
    一键复制
    询价
    优宁维现货
    20μl
    100ul
    100ul 近效期特惠
    选择数量
    当前规格1件起购 
    配送至
    预计3-4个工作日送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价