DRAQ5 ®

品牌： CST

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参数

实验应用：

IF-P/IF-F/IF-IC/F

产品介绍

产品信息

产品详情

Product Usage Information

Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:500 (10 µM) in PBS or Antibody Dilution Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer.Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

组成成分

1,5-双{[2-(二甲氨基) 乙基]氨基}-4, 8-二羟蒽-9,10-二酮 1%~10%，剩余为水

应用

实验应用

IF-P/IF-F/IF-IC/F

背景

DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3). 1.Smith, P.J. et al. (2000) Cytometry 40, 280-91. 2.Wiltshire, M. et al. (2000) Cytometry 39, 217-23. 3.Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.

制备和贮存

保存方式

Store at 4°C protected from light.

参考图片

Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 versus DRAQ5® (DNA content).流式细胞术分析Jurkat细胞，所用抗体为Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 对应 DRAQ5 ® (DNA content)。

Confocal immunofluorescent analysis of mouse testis using Miwi (G82) Antibody #2079 (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5™® (fluorescent DNA dye).激光共聚焦免疫荧光分析小鼠睾丸细胞，所用抗体为Miwi (G82) Antibody #2079 (绿色) 和 Pan-Keratin (C11) Mouse mAb #4545 (红色).蓝色伪彩为荧光DNA染料DRAQ5 ®

Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞，所用抗体为COX IV (3E11) Rabbit mAb #4850 (绿色).肌动蛋白丝采用DY-554 phalloidin (红色)标记。蓝色伪彩为荧光DNA染料DRAQ5 ®

Confocal immunofluorescent analysis of paraffin-embedded human colon adenocarcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).激光共聚焦免疫荧光分析石蜡包埋的人类结肠腺癌细胞，所用抗体为Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 (绿色).蓝色伪彩为荧光DNA染料DRAQ5 ®。

Absorption and fluorescence emission spectra of DRAQ5® in aqueous solution.DRAQ5 ®在水溶性溶液中的吸收和荧光的发射光谱。

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货号：

4084L

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