首页抗体一抗标签抗体

DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody)

1/2

DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody)

品牌： CST

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

反应种属：

All

来源宿主：

Mouse

产品介绍

产品信息

抗原名称

DYKDDDDK Tag

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide.

宿主

Mouse

简单描述

Monoclonal Antibody for studying Flag-Tag. Cited in 466 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, DYKDDDDK Tag (9A3) Mouse Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) (CST #8146) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:200 - 1:800
Immunofluorescence (Immunocytochemistry)	1:800 - 1:1600
Flow Cytometry (Fixed/Permeabilized)	1:400 - 1:1600

应用

反应种属

All

目标/特异性

Specificity/Sensitivity

DYKDDDDK Tag (9A3) Mouse mAb detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma-Aldrich Anti-FLAG M2 antibody.

Species Reactivity:

All Species Expected

敏感性

Transfected Only

背景

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1). 1.Brizzard, B.L. et al. (1994) Biotechniques 16, 730-5.

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #47880.

参考图片

Western blot analysis of extracts from 293T cells, untransfected or transfected with either DYKDDDDK-tagged FoxG1, NPAS4, or Tyrosine Hydroxylase (TH), using DYKDDDDK Tag (9A3) Mouse mAb.Western blot分析293T细胞提取物，未转染或者用DYKDDDDK标记的FoxG1，NPAS4或酪氨酸羟化酶（TH）转染。使用的抗体是：DYKDDDDK Tag (9A3) Mouse mAb。

Confocal immunofluorescent analysis of HeLa cells transfected with a DYKDDDDK-tagged IRF3 construct using DYKDDDDK Tag (9A3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析用DYKDDDDK标记的IRF3质粒转染的HeLa细胞，使用的抗体是：DYKDDDDK Tag (9A3) Mouse mAb（绿色）。肌动蛋白丝用DY-554鬼笔环肽标记（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

Immunohistochemical analysis of paraffin-embedded 293T cell pellets, control (left) or ROS-DYKDDDDK Tag transfected (right), using DYKDDDDK Tag (9A3) Mouse mAb.免疫组化分析石蜡包埋的293T细胞沉淀，对照（左图）或者ROS-DYKDDDDK标签转染的（右图），使用的抗体是：DYKDDDDK Tag (9A3) Mouse mAb。

Flow cytometric analysis of Cos cells, untreated (blue) or transfected with a DYKDDDDK-tagged IRF3 contruct (green) using DYKDDDDK Tag (9A3) Mouse mAb.流式细胞检测分析Cos细胞，未处理（蓝色）或用DYKDDDDK标记的IRF3质粒转染（绿色），使用的抗体是：DYKDDDDK Tag (9A3) Mouse mAb。

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

8146T

一键复制

询价

优宁维现货

20ul

100ul

选择数量

当前规格1件起购

配送至

预计3-4个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价