Enterokinase, Bovine

品牌： UA BIOSCIENCE

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

分子量：

40 kDa(Reducing)

纯度：

>80% by SDS-PAGE

产品介绍

产品信息

耦联标记

Unconjugated

氨基酸序列

Uniprot E6Y432, with C-His Tag

MIVGGSDSREGAWPWVVALYFDDQQVCGASLVSRDWLVSAAHCVYGRNMEPSKWKAVLGLHMASNLTSPQIETRLIDQIVINPHYNKRRKDNDIAMMHLEMKVNYTDYIQPICLPEENQVFSPGRICSIAGWGTLIYQGSTADVLQEADVPLLSNEKCQQQMPEYNITENMVCAGYEAGGVDSCQGDSGGPLMCQENNRWLLAGVTSFGYQCALPNRPGVYARVPRFTEWIQSFLHHHHHHH

分子量

40 kDa(Reducing)

生物活性

2.5U/μl

酶活定义

One unit of Enterokinase cleaves ≥85% of 50 μg control substrate in 16 h at 25°C.

纯度

>80% by SDS-PAGE

内毒素水平

<0.2EU/μg

性状

Liquid

表达系统

Pichia pastoris

缓冲体系

20mM Tris-HCl, pH7.2, 200mM NaCl, 2mM CaCl, 50% Glycerol

标签

His Tag

种属

Bovine

背景

别名

rBoEnterokinase, His; Enteropeptidase; ENTK; PRSS7,重组肠激酶

背景

Enterokinase (enteropeptidase, EC 3.4.4.8) occupies a key position in the utilization of dietary proteins. The enzyme initiates intraluminar digestion of proteins by the proteolytic conversion of trypsinogen to trypsin, which in turn activates the other pancreatic zymogens (Kunitz, 1939a,b; Hadorn et al., 1969). The proteolytic attack of enterokinase is directed exclusively toward the Lys6-Ile7 peptide bond of trypsinogen leaving all other lysine and arginine bonds in the molecule unaffected (Maroux et al., 1971). The resultant cleavage produces the simultaneous release of active trypsin and of the
amino-terminal hexapeptide Val-(Asp)d-Lys (Rovery et al., 1953; Davie and Neurath, 1955). This unique specificity exhibited by enterokinase is of interest as it relates to both the molecular basis of substrate recognition and the control of the digestive process.

制备和贮存

保存方式

· 12 months from date of receipt, -20 to -70 °C as supplied.
· 6 months, -20 to -70 °C under sterile conditions after reconstitution.
· 1 week, 2 to 8 °C under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.

文献

参考图片

The substrate of 50 μ g fusion protein was digested by enzyme, and the addition amount of enterokinase in the sample was 0.5IU, 1IU, 1.5IU and 2IU, respectively. The substrate is a fusion protein with a molecular weight of 10 KD and reacted at 25 ℃ for 16 hours, and the target band of 8.4kD is produced after restriction enzyme digestion.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

UA070001-250IU

一键复制

询价

优宁维现货

250IU

选择数量

当前规格1件起购

配送至

预计2-5个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价