ER-Tracker Green (BODIPY FL Glibenclamide)

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Product Usage Information

ER-Tracker™ Green (BODIPY® FL Glibenclamide) is supplied as a single 100 μg lyophilized vial. To make a 1 mM stock solution, reconstitute the solid in 128 μl of high quality DMSO. Dilute 1:500 directly into normal growth media for a working concentration of 2 μM and analyze immediately. Cells must be imaged live; DO NOT FIX!To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging. BackDrop® Green Background Suppressor #12388 can also be added to reduce background fluorescence.

应用

实验应用

IF-IC

背景

ER-Tracker™ Green (BODIPY® FL Glibenclamide) is a cell-permeant, live cell stain that is highly selective for the endoplasmic reticulum (ER). This stain consists of the green-fluorescent BODIPY® FL dye and glibenclamide. Glibenclamide (glyburide) binds to sulphonylurea receptors, the regulatory subunit of ATP-sensitive K+ channels prominently expressed in the ER. The pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

制备和贮存

保存方式

Store lyophilized solid or solution at –20°C dessicated and protected from light. Once reconstituted in DMSO, the solution should be aliquoted to avoid freeze-thaw cycles. If stored properly, this reagent is stable for 6 months.

参考图片

Confocal immunofluorescent analysis of live HeLa cells using ER-Tracker™ Green (BODIPY® FL Glibenclamide) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析活的HeLa细胞，使用ER-Tracker™ Green (BODIPY® FL Glibenclamide)（绿色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

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货号：

8787S

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100ug

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