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BD Pharmingen™ Purified Mouse Anti-Rat CD32

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BD Pharmingen™ Purified Mouse Anti-Rat CD32

品牌： BD Pharmingen

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参数

反应种属：

Rat (QC Testing)

来源宿主：

Mouse BALB/c IgG1, κ

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产品介绍

产品信息

抗原名称

CD32 (Fc Gamma RII)

宿主

Mouse BALB/c IgG1, κ

免疫原

Recombinant Rat CD32 Protein

简单描述

The D34-485 monoclonal antibody specifically recognizes CD32, the FcγII receptor. Rat CD32 is expressed on B lymphocytes, myeloid cells, and some lymphocytes in the thymic medulla. D34-485 antibody blocks binding of aggregated immunoglobulins to the FcγII receptors in vitro. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

商品描述

同种型

Mouse BALB/c IgG1, κ

克隆号

克隆 D34-485 (RUO)

浓度

0.5 mg/ml

产品详情

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

应用

实验应用

Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed, Western blot (Reported), Immunohistochemistry-paraffin (Not Recommended)

反应种属

Rat (QC Testing)

目标/特异性

CD32

背景

别名

Fcgr2b; FcRII; Fc-gamma RII; FcγRII; FcγII receptor

制备和贮存

存储溶液

Aqueous buffered solution containing ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing ≤0.09% sodium azide.

参考图片

Blocking of Fc-mediated binding to FcγII receptors (CD32) on rat splenocytes. Lewis rat splenocytes were pre-incubated with purified isotype control mAb A112-2 (Cat. No. 553487, left panel) or Rat BD Fc Block™ purified anti-rat CD32 mAb D34-485 (right panel). Two-color staining was performed with FITC-conjugated anti-rat NKR-P1A mAb 10/78 (Cat. No. 555008) and PE-conjugated anti-rat CD45RA mAb OX-33 (Cat. No. 551402/554884). Note how the dim staining of B lymphocytes (OX-33+ cells) by anti-CD161a (NKR-P1A) (left panel) is reduced when Rat BD Fc Block™ is used before staining (right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

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货号：

550271

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