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rhFGF-10, CF (25 ug)
rhFGF-10, CF (25 ug)
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rhFGF-10, CF (25 ug)

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品牌： R&D Systems
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    Scientific Data

    Bioactivity Recombinant Human FGF-10 Protein BioactivityView Larger

    Recombinant Human FGF-10 (Catalog # 345-FG) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50 for this effect is 20-100 ng/mL.

    SDS-PAGE Recombinant Human FGF-10 Protein SDS-PAGEView Larger

    1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.

    Bioactivity View Larger

    Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and cultured for 20-60 days in lung organoid expansion medium, which includes Recombinant Human FGF-10 (Catalog # 345-FG), Recombinant Human KGF/FGF-7 (251-KG), Recombinant Human Noggin (6057-NG), and Recombinant Human R-Spondin 1 (4645-RS), along with the other reagents listed in the lung organoid expansion medium recipe in the lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog # NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (MAB2018; red). All samples were counterstained with DAPI (5748; blue).

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    345-FG

    FormulationLyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    345-FG/CF

    FormulationLyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Human FGF-10 Protein Summary

    Product Specifications

    Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 20-100 ng/mL.
    Source
    E. coli-derived human FGF-10 protein
    Cys37-Ser208 & Gly41-Ser208
    Accession #
    O15520
    N-terminal Sequence
    Analysis
    Cys37 & Gly41
    Predicted Molecular Mass
    19.5 kDa
    SDS-PAGE
    19-22 kDa, reducing conditions
    背景
    背景

    Background: FGF-10

    The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (8, 9), formation of the otic vesicle and chochlea (10), adipogenesis (11), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (12 - 15). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 10, 13 ‑ 15). The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung (8, 9). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF‑10 biology (7, 10, 14, 15). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (16).

    References
    1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
    2. Igarashi, M. et al. (1998) J. Biol. Chem. 273:13230.
    3. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
    4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
    5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
    6. Min, H. et al. (1998) Genes Dev. 12:3156.
    7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
    8. Bellusci, S. et al. (1997) Development 124:4867.
    9. Weaver, M. et al. (2000) Development 127:2695.
    10. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
    11. Sakaue, H. et al. (2002) Genes Dev. 16:908.
    12. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
    13. Mailleux, A.A. et al. (2002) Development 129:53.
    14. Makarenkova, H.P. et al. (2000) Development 127:2563.
    15. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
    16. Nomura, S. et al. (2008) Br. J. Cancer 99:305.
    Long Name
    Fibroblast Growth Factor 10
    Entrez Gene IDs
    2255 (Human); 14165 (Mouse); 25443 (Rat)
    Alternate Names
    FGF10; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; KGF2; KGF-2; produced by fibroblasts of urinary bladder lamina propria
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

    参考图片

    1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.

    Recombinant Human FGF-10 (Catalog # 345-FG) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50 for this effect is typically 20‑100 ng/mL.

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    货号：
    345-FG-025/CF
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