Phospho-Fgr (Tyr412) Antibody

Fgr (c-Fgr kinase) is a member of the Src tyrosine kinase family. It has a membrane-associated amino-terminal domain that is highly divergent from other family members, internal conserved SH2 and SH3 domains, and a highly conserved carboxy-terminal tyrosine kinase catalytic domain (1,2). Tyrosine 412 is located in the activation loop; autophosphorylation of this residue is critical for the activation of Fgr tyrosine kinase activity, and is counter-regulated by Src-mediated phosphorylation at Tyr523 (3). c-Fgr is predominantly expressed in cells of hematopoietic origin, including differentiated myeloid cells, NK, and B cells (4,5). Fgr plays an important role in the signaling cascade from membrane receptors lacking intrinsic tyrosine kinase activity, such as Bcr, FcR, and the integrin family of receptors (6). It was demonstrated that Fgr functions as a selective inhibitor of beta 2 integrin-mediated signaling and Syk kinase function in monocytes (6). 1.Willman, C.L. et al. (1991) Blood 77, 726-734. 2.Patel, M. et al. (1991) Pathobiology 59, 289-292. 3.Ruzzene, M. et al. (1994) J Biol Chem 269, 15885-91. 4.Wechsler, R.J. and Monroe, J.G. (1995) J Immunol 154, 3234-44. 5.Notario, V. et al. (1989) J Cell Biol 109, 3129-36. 6.Vines, C.M. et al. (2001) Immunity 15, 507-19.