FICOLL PM400 5KG

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FICOLL PM400 5KG

品牌： Cytiva (GE Life)

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实验应用：

Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes.
It also has applications in defined cu

产品介绍

产品信息

产品详情

一种平均分子量为 400000 的高度稳定性蔗糖聚合物，适用于分离真核细胞和细胞器，并且损坏极少。用于产生密度梯度，以通过离心沉淀或通过在单位重力下的沉降来分离细胞和亚细胞成分。最高浓度可达 50％ (w/v)，覆盖密度最高可达 1.2 g/mL。渗透性能优于蔗糖。保持功能和形态完整性。用于分离对离心沉淀敏感的细胞，以及用于分离密度相似但大小不同的细胞（在单位重力沉降条件下）。非渗透性生物膜。用作制备 Ficoll-Paque 梯度的原料。用于其他应用，例如电泳、杂交、冷冻保存以及用作半抗原载体。密度高而不增加渗透压Ficoll PM400 是合成的中性、高度支化亲水性蔗糖聚合物，平均分子量为 400000。长期以来，它一直被用来形成密度梯度，用于分离和分离真核细胞、细胞器和细菌细胞，作为稳定剂和分离单核细胞的制备培养基。相关应用还可以参见所定义的培养基、核酸杂交、电泳和免疫学研究。高度可溶通过将蔗糖与环氧氯丙烷共聚而形成的高分子量蔗糖聚合物。分子高度支化，羟基含量高，使得在水性培养基中溶解度非常好。

产品用途

一种高度稳定的蔗糖聚合物，平均分子量为 400000，用于分离细胞和细胞器。

商品描述

Ficoll PM 400 密度梯度分离液一种平均分子量为 400000 的高度稳定性蔗糖聚合物，适用于分离真核细胞和细胞器，并且损坏极少。

外观

White

形状

固体

颜色

白色

最大密度

1.2 g/ml

产品参数

Application

Cell isolation
Density Max.

1.2 g/ml
Endotoxin Activity Max.

0.12 EU/mg
Estimated shelf life from manufacture date

5 years
Storage conditions

4–30°C
Stability

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Physical State

Solid
Physical Form

Spray-dried powder
Molecular Weight (Mr) Max.

~ 5 × 10^{~ 5}
Molecular Weight (Mr) Min.

~ 3 × 10⁵
Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll PM 400 can be sterilized by autoclaving at 110°C for 30 min without degradation. Strong oxidizing and reducing agents should be avoided
Stokes Radius

~ 10
Color

White
Specific Rotation (alpha)20

50°-65°
Relative Viscosity

5 ηr at 20°C: 10% (w/v) 20 ηr at 20°C: 20% (w/v) 60 ηr at 20°C : 30% (w/v 180 ηr at 20°C : 40% (w/v) 600 ηr at 20°C : 50% (w/v)
Intrinsic Viscosity

~ 17
Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained
Certificate of Analysis

Yes

组成成分

100%聚蔗糖

应用

实验应用

制备和贮存

保存

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

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货号：

17030005

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