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BD Pharmingen™ FITC BrdU Flow Kit


品牌: BD Pharmingen








实验应用:
Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)
Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)
产品介绍
产品信息
抗原名称
BrdU

克隆号
(RUO)

组合货号
51-2354AK/(2 to 8C)+51-2354BK/(max.-70C)

应用
实验应用
Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)

目标/特异性
BrdU

文献
文献
研发参考(27)
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4. Endl E, Steinbach P, Knüchel R, Hofstädter F. Analysis of cell cycle-related Ki-67 and p120 expression by flow cytometric BrdUrd-Hoechst/ 7AAD and immunolabeling technique. Cytometry. 1997; 29:233-241. (Biology).
5. Gonchoroff NJ, Katzmann JA, Currie RM, et al. S-phase detection with an antibody to bromodeoxyuridine. Role of DNase pretreatment. J Immunol Meth. 1986; 93:97-101. (Biology).
6. Gratzner HG, Leif RC. An immunofluorescence method for monitoring DNA synthesis by flow cytometry. Cytometry. 1981; 1:385-393. (Biology).
7. Gray JW, Darzynkiewicz Z,, ed. Techniques in cell cycle analysis,. Clifton, New Jersey: Humana Press,;
8. Holm M, Thomsen M, Hoyer M, Hokland P. Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells. Cytometry. 1998; 32(1):28-36. (Biology).
9. Houck DW, Loken MR. Simultaneous analysis of cell surface antigens, bromodeoxyuridine incorporation and DNA content. Cytometry. 1985; 6:531-538. (Biology).
10. Lacombe F, Belloc F, Bernard P, Boisseau MR. Evaluation of four methods of DNA distribution data analysis based on bromodeoxyuridine/DNA bivariate data. Cytometry. 1988; 9:245-253. (Biology).
11. Lakhanpal S, Gonchoroff NJ, Katzmann JA, Handwerger BS. A flow cytofluorometric double staining technique for simultaneous determination of human mononuclear cell surface phenotype and cell cycle phase. J Immunol Meth. 1987; 96:35-40. (Biology).
12. Mehta BA, Maino VC. Simultaneous detection of DNA synthesis and cytokine production in staphylococcal enterotoxin B activated CD4+ T lymphocytes by flow cytometry. J Immunol Methods. 1997; 208:49-59. (Biology).
13. Miltenburger HG, Sachse G, Schliermann M. S-phase cell detection with a monoclonal antibody. Dev Biol Stand. 1987; 66:91-99. (Biology).
14. Moran R, Darzynkiewicz Z, Staiano-Coico L, Melamed MR. Detection of 5-bromodeoxyuridine (BrdUrd) incorporation by monoclonal antibodies: role of the DNA denaturation step. J Histochem Cytochem. 1985; 33:821-827. (Biology).
15. Penit C. In vivo thymocyte maturation. BrdU labeling of cycling thymocytes and phenotypic analysis of their progeny support the single lineage model. J Immunol. 1986; 137:2115-2121. (Biology).
16. Robey E, Chang D, Itano A, et al. An activated form of Notch influences the choice between CD4 and CD8 T cell lineages. Cell. 1996; 87:483-492. (Biology).
17. Rocha B, Penit C, Baron C, Vasseur F, Dautigny N, Freitas AA. Accumulation of bromodeoxyuridine-labeled cells in central and peripheral lymphoid organs: minimal estimates of production and turnover rates of mature lymphocytes. Eur J Immunol. 1990; 20(8):1697-1708. (Biology).
18. Sasaki K, Adachi S, Yamamoto T, Murakami T, Tanaka K, Takahashi M. Effects of denaturation with HCl on the immunological staining of bromodeoxyuridine incorporated into DNA. Cytometry. 1988; 9:93-96. (Biology).
19. Sasaki K, Murakami T, Takahashi M. Flow cytometric analysis of cell proliferation kinetics using the anti-BrdUrd antibody. Gan To Kagaku Ryoho. 1989; 16:2338-2344. (Biology).
20. Schittek B, Rajewsky K, Forster I. Dividing cells in bone marrow and spleen incorporate bromodeoxyuridine with high efficiency. Eur J Immunol. 1991; 21:235-238. (Biology).
21. Shapiro, HM. Practical Flow Cytometry. (Biology).
22. Thoman ML. Early steps in T cell development are affected by aging. Cell Immunol. 1997; 178(2):117-123. (Biology).
23. Toba K, Winton EF, Bray RA. Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation. Cytometry. 1992; 13:60-67. (Biology).
24. Tough DF, Sprent J. Turnover of naive- and memoryphenotype T cells. J Exp Med. 1994; 179:1127-1135. (Biology).
25. Vanderlaan M, Thomas CB. Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 1985; 6:501-505. (Biology).
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参考图片
The measurement of cell-incorporated BrdU (with anti-BrdU FITC) and total DNA content (with 7-AAD) in D10.G4.1 cells. The D10.G4.1 cells were cultured with 10 μM of BrdU for 30 minutes. As shown by the regions applied to the 7-AAD vs BrdU dot plot, flow cytometric analysis of cells stained with the reagents provided in the BrdU Flow Kit allowed for the discrimination of cell subsets that were apoptotic—defined as sub-G0/G1 (R6, 5.6% of cells) or resided in G0/G1 (R3, 38.6%), S (R4, 38.6%), or G2 + M (R5, 14.4%) phases of the cell cycle and had recently synthesized DNA.5,6 The 7-AAD signal data is displayed on a linear scale, as shown on the x-axis.
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