Fixation/Permeabilization Solution Kit with BD Golgigtop

The BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit provides Fixation/Permeabilization solution, BD Perm/Wash™ Buffer, and BD GolgiStop™ protein transport inhibitor containing monensin. The ex vivo addition of BD GolgiStop™ to in vitro- or in vivo-stimulated cells blocks their intracellular transport processes. This results in the accumulation of most cytokine proteins in the Golgi complex and thereby enhances cytokine staining signals. Enough BD GolgiStop reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 10 ⁶ cells/ml. Components Quantity  Fixation/Permeabilization solution  125 mL  BD Perm/Wash™ Buffer (10x)  100 mL  BD GolgiStop protein transport inhibitor containing monensin  0.7 mL

The BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit provides Fixation/Permeabilization solution, BD Perm/Wash™ Buffer, and BD GolgiStop™ protein transport inhibitor containing monensin. The ex vivo addition of BD GolgiStop™ to in vitro- or in vivo-stimulated cells blocks their intracellular transport processes. This results in the accumulation of most cytokine proteins in the Golgi complex and thereby enhances cytokine staining signals. Enough BD GolgiStop reagent is provided for treating up to 1 liter of cell culture at a cell density of up to 2 × 10 ⁶ cells/ml. Components Quantity  Fixation/Permeabilization solution  125 mL  BD Perm/Wash™ Buffer (10x)  100 mL  BD GolgiStop protein transport inhibitor containing monensin  0.7 mL

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