FLAG Tag Recombinant Rabbit mAb (S-589-8)

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FLAG Tag Recombinant Rabbit mAb (S-589-8)

品牌： STARTER

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参数

反应种属：

Species Independent

来源宿主：

Rabbit

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产品介绍

产品信息

耦联标记

Unconjugated

纯化方式

Protein A

宿主

Rabbit

免疫原

Synthetic Peptide

同种型

IgG

克隆号

S-589-8

浓度

0.5 mg/ml

性状

Liquid

缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

产品类型

Recombinant mAb

应用

实验应用

WB、ICC

反应种属

Species Independent

稀释度

WB

1:1000-1:10000
ICC

1:500

背景

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine). It is one of the most specific tags and it is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins within living cells. FLAG-tag has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. FLAG-tag can also be used in the isolation of protein complexes with multiple subunits.

制备和贮存

保存方式

12 months from date of receipt / reconstitution, -20 °C as supplied

参考图片

WB result of FLAG Tag Rabbit mAb
Primary antibody: FLAG Tag Rabbit mAb at 1/1000 dilution
Lane 1: 293-F whole cell lysate 20 µg
Lane 2: 293-F transfected with Flag tagged GFP whole cell Lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 32 kDa

WB result of FLAG Tag Rabbit mAb
Primary antibody: FLAG Tag Rabbit mAb at 1/1000 dilution
Lane 1: Nicotiana benthamiana leaves (transfected with HA tagged GFP) lysate 20 µg
Lane 2: Nicotiana benthamiana leaves (transfected with Flag tagged GFP) lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 35 kDa
(This blot was developed with high sensitivity substrate)

ICC shows positive staining in Histone H3-Flag-V5 tag transfected HeLa cells (top panel) and negative staining in vector-transfected HeLa cells (below panel). Anti-FLAG Tag antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

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货号：

S0B0573-1ml

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