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GAPDH (D16H11) XP ® Rabbit mAb

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GAPDH (D16H11) XP ® Rabbit mAb

品牌： CST

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参数

分子量：

反应种属：

Human,Mouse,Rat,Monkey,

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产品介绍

产品信息

抗原名称

GAPDH

来源纯化

Monoclonal antibody is produced by immunzing animals with a synthetic peptide corresponding to residues surrounding Lys260 of human GAPDH protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying GAPDH. Cited in 6310 publications. Validated for WB, WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, GAPDH (D16H11) XP® Rabbit Monoclonal Antibody (CST #5174) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunohistochemistry (Paraffin)	1:800
Immunofluorescence (Immunocytochemistry)	1:200

分子量

内毒素水平

猪

研究领域

癌症,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,Monkey,

预测反应种属

Pig

目标/特异性

Specificity/Sensitivity

GAPDH (D16H11) XP® Rabbit mAb detects endogenous levels of total GAPDH protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5). 1.Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95. 2.Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38. 3.Zheng, L. et al. (2003) Cell 114, 255-66. 4.Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9. 5.Wang, Q. et al. (2005) FASEB J. 19, 869-71. 6.Kozako, T. et al. (2015) Sci Rep 5, 11345.

研究领域

癌症,代谢,神经科学

翻译后修饰

Unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #92310.

数据库链接

Entrez-Gene ID

2597

UniProt ID

P04406

参考图片

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb.

免疫组化检测人乳腺癌石蜡切片，所用抗体为GAPDH (D16H11) XP® Rabbit mAb兔单抗

Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb.

Wetern blot检测多种细胞系提取物，所用抗体为GAPDH (D16H11) XP® Rabbit mAb兔单抗。

Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦免疫荧光检测C2C12细胞提取物，所用抗体为GAPDH (D16H11) XP® Rabbit mAb兔单抗（绿色），微丝用DY-554 phalloidin染色(红色)。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084)

Immunohistochemical analysis of paraffin-embedded mouse colon using GAPDH (D16H11) XP® Rabbit mAb.

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货号：

5174T

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