GAPDH Recombinant Rabbit mAb (S-240-147)

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GAPDH Recombinant Rabbit mAb (S-240-147)

品牌： STARTER

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参数

反应种属：

Hu、Ms、Rat、Zebrafish

来源宿主：

Rabbit

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产品介绍

产品信息

耦联标记

Unconjugated

纯化方式

Protein A

抗原名称

GAPDH

宿主

Rabbit

免疫原

Synthetic Peptide

克隆号

S-240-147

浓度

1 mg/ml

性状

Liquid

缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

产品类型

Rabbit mAb

应用

实验应用

WB、IHC-P、ICC

反应种属

Hu、Ms、Rat、Zebrafish

预测反应种属

Hamster (95%), Mink (95%), Pig (95%), Squirrel (95%),Dog (95%), Cat (95%), Sheep (95%), Quail (95%), Bovine (95%), Chicken (95%)

稀释度

WB

1:10000-1:50000
IHC

1:5000
ICC

1:50-1:200

背景

别名

Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

背景

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules [PMID: 17072346]. GAPDH comprises a polypeptide chain of 335 amino acids. Structural studies identified two regions, namely the glyceraldehyde-3-phosphate catalytic site and the NAD+ binding site, a primary structure known as the Rossmann fold, which is also required for the activity of other dehydrogenases. Many of these roles are dependent on the ability of GAPDH to bind different macromolecules in the cell.

细胞定位

Cytoplasm、Nucleus、Membrane

制备和贮存

保存方式

12 months from date of receipt / reconstitution, -20 °C as supplied

数据库链接

Accession

P04406，P16858

参考图片

WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/10000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: THP-1 whole cell lysate 20 µg Lane 3: MCF7 whole cell lysate 20 µg Lane 4: NIH/3T3 whole cell lysate 20 µg Lane 5: mouse spleen lysate 20 µg Lane 6: C6 whole cell lysate 20 µg Lane 7: rat spleen lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa Exposure time: 180 s

WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/50000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: THP-1 whole cell lysate 20 µg Lane 3: MCF7 whole cell lysate 20 µg Lane 4: NIH/3T3 whole cell lysate 20 µg Lane 5: mouse spleen lysate 20 µg Lane 6: C6 whole cell lysate 20 µg Lane 7: rat spleen lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa Exposure time: 180 s

IHC shows positive staining in paraffin-embedded human spleen. Anti-GAPDH antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-GAPDH antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-GAPDH antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

WB result of GAPDH Rabbit mAb
Primary antibody: GAPDH Rabbit mAb at 1/10000 dilution
Lane 1: zebrafish lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa
Exposure time: 60 s

WB result of GAPDH Rabbit mAb
Primary antibody: GAPDH Rabbit mAb at 1/50000 dilution
Lane 1: zebrafish lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa
Exposure time: 60 s

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货号：

S0B0261-1ml

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