GABARAP (E1J4E) Rabbit mAb

GABAA receptor associated protein (GABARAP) is an Atg8 family protein with a key role in autophagy, which was originally discovered as a protein associated with the GABAA receptor regulating receptor trafficking to the plasma membrane (1). Proteins in this family, including microtubule-associated protein light chain 3 (LC3) and GATE-16 (GABARAPL2), become incorporated into the autophagosomal membranes following autophagic stimuli such as starvation (2). Like the other family members, GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine (3,4). This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members (5,6) followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3 (7,8). GABARAPL1/GEC1, a protein that is highly related to GABARAP, was identified as an estrogen inducible gene, and is also associated with autophagosomes (9-11). 1.Wang, H. et al. (1999) Nature 397, 69-72. 2.Shpilka, T. et al. (2011) Genome Biol 12, 226. 3.Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12. 4.Sou, Y.S. et al. (2006) J Biol Chem 281, 3017-24. 5.Tanida, I. et al. (2004) J Biol Chem 279, 36268-76. 6.Hemelaar, J. et al. (2003) J Biol Chem 278, 51841-50. 7.Tanida, I. et al. (2001) J Biol Chem 276, 1701-6. 8.Tanida, I. et al. (2002) J Biol Chem 277, 13739-44. 9.Chakrama, F.Z. et al. (2010) Autophagy 6, 495-505. 10.Pellerin, I. et al. (1993) Mol Cell Endocrinol 90, R17-21. 11.Vernier-Magnin, S. et al. (2001) Biochem Biophys Res Commun 284, 118-25.