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rhGDNF (10 ug)
rhGDNF (10 ug)
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rhGDNF (10 ug)

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品牌： R&D Systems
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    产品介绍
    产品信息

    Scientific Data

    SEC-MALS View Larger

    Recombinant human GDNF (Catalog # 212-GD) has a molecular weight (MW) of 27.5 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

    Bioactivity Recombinant Human GDNF Protein BioactivityView Larger

    Recombinant Human GDNF (Catalog # 212-GD) induces SH-SY5Y human neuroblastoma cell proliferation in the presence of Recombinant Human GFRa-1 Fc Chimera (Catalog # 714-GR). The activity is approximately 9-fold greater than the top competitor's GDNF.

    SDS-PAGE Recombinant Human GDNF Protein SDS-PAGEView Larger

    1 μg/lane of Recombinant Human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 17 kDa and 34 kDa, respectively.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    212-GD

    FormulationLyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    212-GD/CF

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Human GDNF Protein Summary

    Product Specifications

    Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714‑GR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
    Source
    Mouse myeloma cell line, NS0-derived human GDNF protein
    Arg109-Ile211
    Accession #
    P39905
    N-terminal Sequence
    Analysis
    Arg109
    Structure / Form
    Disulfide-linked homodimer
    Predicted Molecular Mass
    11.6 kDa (monomer)
    背景
    别名
    Astrocyte-derived trophic factor,ATF,ATF1,ATF2,GDNF,glial cell derived neurotrophic factor,glial cell line derived neurotrophic factor,glial cell line-derived neurotrophic factor,HFB1-GDNF,HGDNF,HSCR3,Glial Cell line-derived Growth Factor
    背景

    Background: GDNF

    Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

    Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

    Long Name
    Glial Cell line-derived Growth Factor
    Entrez Gene IDs
    2668 (Human); 25453 (Rat)
    Alternate Names
    Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

    参考图片

    1 ug/lane of Recombinant Human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 17 kDa and 34 kDa, respectively.

    Recombinant Human GDNF (Catalog # 212-GD) stimulates proliferation in the SH‑SY5Y human neuroblastoma cell line. The ED50 for this effect is typically 2-12 ng/mL in the presence of Recombinant Human
    GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR).

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    货号：
    212-GD-010
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