Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate)
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Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate)

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    反应种属:
    Ms
    Ms
    来源宿主:
    Goat
    Goat
    展开
    产品介绍
    产品信息
    耦联标记
    Alexa Fluor® 488
    纯化方式
    Immunogen Affinity
    宿主
    Goat
    浓度
    2 mg/ml
    性状
    Liquid
    缓冲体系
    PBS, 1% BSA, 0.3% Proclin 300
    产品类型
    Polyclonal antibody
    应用
    实验应用
    ICC、FCM、IF
    反应种属
    Ms
    稀释度
    • ICC

      1:500
    • IF

      1:500
    • FCM

      1:2000
    制备和贮存
    保存方式

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

    参考图片

    ICC shows positive staining in Raji cells. Anti-CD19 antibody (S0B2311) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    Negative control: ICC shows negative staining in Jurkat cells. Anti-CD19 antibody (S0B2311) was used at 1/500 dilution and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    IF shows positive staining in paraffin-embedded human spleen. Anti-CD19 antibody (S0B2311) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    IF shows positive staining in paraffin-embedded human tonsil. Anti-CD19 (S0B2311) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    IF shows positive staining in paraffin-embedded human diffuse large B cell lymphoma. Anti-CD19 antibody (S0B2311) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte, left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling CD28 antibody (S0B0009) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Mouse IgG(H+L) (Alexa Fluor® 488 Conjugate) antibody at 1/2000 (0.1 μg) dilution was used as the secondary antibody.
    Negative control: THP-1

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    货号:
    S0B4017-1ml
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