Goat anti-Rabbit IgG(H+L) (Alexa Fluor® 594 Conjugate)

ICC shows positive staining in HeLa cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

ICC shows positive staining in MCF7 cells. Anti-HSP60 antibody (S0B0002) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

ICC shows positive staining in HeLa cells. Anti-α-tubulin antibody (S0B0043) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

ICC shows positive staining in MCF7 cells. Anti-α-tubulin antibody (S0B0043) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG (H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/500 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).