Goat Polyclonal Goat anti-Mouse IgG3 Secondary Antibody

Unconjugated

Ion exchange chromatography

Goat

Pools of purified homogenous IgG3 isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

LYOPH

No Preservative

Polyclonal

IGHG3

Dot Blot ELISA Western Blot

MuSpecies Glossary

The reactivity of the antiserum is directed to the subclass IgG3. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion.