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简单描述
Cellular Dyes for studying Hoechst in the research area.
商品描述
Product Usage Information
This product is soluble up to 50 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 μg/ml.
组成成分
三盐酸-2-(4-乙氧苯基)-5-(4-甲基-1-哌嗪基)-2,5双-1H-苯并咪唑 60%-100%，乙腈1%-5%
应用
实验应用
IF-P/IF-F/IF-IC
背景
背景
Hoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double-stranded AT-rich regions. This stain can be used on both live and fixed cells (1).
1.Portugal, J. and Waring, M.J. (1988) Biochim Biophys Acta 949, 158-68.
制备和贮存
保存方式
The lyophilized powder is stable for 1 year if kept below 0°C with desiccant. Aqueous solutions are stable for at least 1 month if kept in the dark between 2-8°C.
参考图片
Immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb #3873 (red) and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (green). Blue pseudocolor = Hoechst 33342 (fluorescent DNA dye).免疫荧光分析HeLa细胞，所用抗体为α-Tubulin (DM1A) Mouse mAb #3873 (红色）和 Phospho-Histone H3 (Ser10) (D2C8) XP™ Rabbit mAb #3377 (绿色)，蓝色伪彩为Hoechst 33342（荧光DNA染料）
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