






Product Usage Information
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent assays:Immunofluorescent: 1:500 - 1:2000


Specificity/Sensitivity
Species Reactivity:
Rat


参考图片
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or heat shocked (right), using HSP70 (6B3) Rat mAb #4873 detected with Anti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦免疫荧光分析HeLa细胞,未处理组(左图),右图为热休克后的,所用抗体为HSP70 (6B3) Rat mAb #4873,检测抗体采用Anti-Rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (绿色)。肌动蛋白丝采用Alexa Fluor ® 555 phalloidin (红色)。
Flow cytometric analysis of HeLa cells using HSP70 (6B3) Rat mAb #4873 detected with Anti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate) (green) and compared to a nonspecific negative control antibody (red).流式细胞术分析HeLa细胞,所用抗体为HSP70 (6B3) Rat mAb #4873,检测抗体采用Anti-Rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (绿色)与非特异性阴性对照抗体作比较(红色)。