Anti-rabbit IgG (H+L), F(ab‘) 2 Fragment (Alexa Fluor ® 488 Conjugate)

Confocal immunofluorescent analysis of P19 cells using LIN28A (A177) Antibody #3978 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析P19细胞，所用抗体为LIN28A (A177) Antibody #3978检测抗体为anti- Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 488 Conjugate) (绿色)。肌动蛋白丝采用DY-554 phalloidin (红色)，蓝色伪彩为荧光DNA染料，信息为DRAQ5 ® #4084。

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951 and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060 detected with Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) and compared to a nonspecific negative control antibody (red).流式细胞术分析Jurkat细胞，绿色是未处理组，蓝色为采用LY294002 #9901, Wortmannin #9951 和 U0126 #9903 (blue)处理组。所用抗体为Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060，检测抗体为Anti-Rabbit IgG (H+L), F(ab’)2Fragment (Alexa Fluor ® 488 Conjugate) (绿色) ，与非特异性阴性对照抗体（红色）作对比。

High content analysis of HeLa cells exposed to varying concentrations of staurosporine for 3hr. With increasing concentrations of staurosporine, a significant decrease (~2.5 fold) in phospho-MAPKAPK-2 signal as compared to the untreated control was observed. When using phospho-MAPKAPK-2 as a measurement, the IC50 of this compound was 92.5 mM. Data were generated on the Acumen HCS platform using Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate).高通量分析HeLa细胞，该细胞暴露于不同浓度的十字孢碱3个小时。与未处理的对照组相比较，随着十字孢碱浓度的增加phospho-MAPKAPK-2信号显著降低（约2.5倍）。当采用 phospho-MAPKAPK-2检测时，IC50为92.5mM。数据时以Acumen ® HCS为平台生成的，所用抗体为Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 488 Conjugate)。