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Anti-rabbit IgG (H+L), F(ab‘) 2 Fragment (Alexa Fluor ® 594 Conjugate)

品牌： CST

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对比

咨询

参数

反应种属：

Rabbit

来源宿主：

Goat

产品介绍

产品信息

耦联标记

Alexa Fluor 594

抗原名称

IgG

宿主

Goat

简单描述

Secondary Antibody for studying Rabbit IgG in the research area.

商品描述

Product Usage Information

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 – 1:2000 should yield acceptable results for immunofluorescent and flow cytometry assays.

应用

反应种属

Rabbit

目标/特异性

Specificity/Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins, and purified human paraproteins.

Species Reactivity:

Rabbit

背景

This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

制备和贮存

保存方式

Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

参考图片

Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞，使用COX IV (3E11) Rabbit mAb #4850检测Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate).流式细胞检测分析Jurkat细胞，未处理（绿色）或用LY294002 #9901、Wortmannin #9951和U0126 #9903（蓝色）处理，使用Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060检测Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)。

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货号：

8889S

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询价

供应商现货

250ul

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