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Anti-mouse IgG (H+L), F(ab‘) 2 Fragment (Alexa Fluor ® 594 Conjugate)

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Anti-mouse IgG (H+L), F(ab‘) 2 Fragment (Alexa Fluor ® 594 Conjugate)

品牌： CST

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对比

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参数

反应种属：

Mouse

来源宿主：

Goat

产品介绍

产品信息

耦联标记

Alexa Fluor 594

抗原名称

IgG

宿主

Goat

简单描述

Secondary Antibody for studying Mouse IgG in the research area.

商品描述

Product Usage Information

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 - 1:2000 should yield acceptable results for immunofluorescent and flow cytometry assays.

应用

反应种属

Mouse

目标/特异性

Specificity/Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum. Cross-reactivity with rat primary antibodies has been observed.

Species Reactivity:

Mouse

背景

This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

制备和贮存

保存方式

Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

参考图片

Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red).流式细胞检测分析未处理的Jurkat细胞，使用Akt (5G3) Mouse mAb #2966检测Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)（蓝色），同时用Mouse (G3A1) mAb IgG1 Isotype Control #5415（红色）作为同型对照。

Immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).免疫荧光分析HeLa细胞，使用β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677检测Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)（红色）。蓝色假色= DRAQ5® #4084（DNA荧光染料）。

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货号：

8890S

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询价

供应商现货

250ul

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