Anti-mouse IgG (H+L), F(ab‘) 2 Fragment (Alexa Fluor ® 647 Conjugate)

Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).流式细胞术分析未处理的Jurkat细胞，所用抗体为Akt (5G3) Mouse mAb #2966，检测抗体为Anti-Mouse IgG (H+L), F(ab’)2Fragment (Alexa Fluor ® 647 Conjugate) (绿色) ，与非特异性阴性对照抗体（红色）作对比。

High content analysis of C2C12 cells exposed to varying concentrations of U0126 #9903 for 2 hours. With increasing concentrations of U0126, a significant decrease (~20 fold) in phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) signal as compared to the untreated control was observed. When using Phospho-p44/42 MAPK (Erk1/2) protein as a measurement, the IC50 of this compound was 2.5 μM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate).高通量分析C2C12细胞，该细胞暴露于LY294002 #9901 2个小时。与未处理的对照组相比较，随着LY294002 浓度的增加phospho-S6 Ribosomal protein (Ser202/204)信号显著降低（约20倍）。当采用 Phospho-p44/42 MAPK (Erk1/2)检测时，IC50为2.5mM。数据时以Acumen ® HCS为平台生成的，所用抗体为Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 647 Conjugate)。

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb #4545 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞，所用抗体为g Pan-Keratin (C11) Mouse mAb #4545检测抗体为anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 647 Conjugate) (蓝色)。 红色为propidium iodide，荧光DNA染料。