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Mouse (E7Q5L) mAb IgG2b Isotype Control
Mouse (E7Q5L) mAb IgG2b Isotype Control

Mouse (E7Q5L) mAb IgG2b Isotype Control

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品牌： CST
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    来源宿主：
    Mouse
    Mouse
    产品介绍
    产品信息
    宿主
    Mouse
    简单描述
    Monoclonal Antibody for studying Mouse IgG isotype control. Cited in 12 publications. Validated for IP, IHC, IF, IF, F, F, ChIP. Highly specific and rigorously validated in-house, Mouse (E7Q5L) Monoclonal Antibody IgG2b Isotype Control (CST #53484) is ready to ship.
    商品描述

    Product Usage Information

    Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.

    浓度
    2.5 mg/ml
    应用
    目标/特异性

    Specificity/Sensitivity

    Mouse (E7Q5L) mAb IgG2b Isotype Control is not directed against any known antigen. It functions as an isotype control for mouse IgG2b monoclonal antibodies.

    敏感性
    Endogenous
    背景
    背景
    Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    参考图片

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using p53 (DO-7) Mouse mAb #48818 (left) compared to concentration matched Mouse (E7Q5L) mAb IgG2b Isotype Control (right).

    Confocal immunofluorescent analysis of HCT 116 cells using β-Actin (8H10D10) Mouse mAb #3700 (left, green) compared to concentration matched Mouse (E7Q5L) mAb IgG2b Isotype Control (right, green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

    Flow cytometric analysis of HeLa cells using β-Actin (8H10D10) Mouse mAb #3700 (solid line) compared to concentration-matched Mouse (E7Q5L) mAb IgG2b Isotype Control (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells treated with UV (1000 J/m2 followed by a 3 hour recovery) and either Mouse (E7Q5L) mAb IgG2b Isotype Control, Normal Rabbit IgG #2729, or p53 (DO-7) Mouse mAb #48818 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Promoter Primers #6449, SimpleChIP® Human MDM2 Intron 2 Primers #90678, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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    货号：
    53484S
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    询价
    供应商现货
    100ul
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