全部商品分类
用小程序，查商品更便捷
Product Usage Information
Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.
Specificity/Sensitivity
参考图片
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using PCNA (PC10) Mouse mAb (left) compared to concentration matched Mouse (E5Y6Q) mAb IgG2a Isotype Control (right).
Confocal immunofluorescent analysis of HCT 116 cells using CD44 (156-3C11) Mouse mAb #3570 (left, green) compared to concentration matched Mouse (E5Y6Q) mAb IgG2a Isotype Control (right, green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.
Flow cytometric analysis of unpermeabilized HeLa cells using CD44 (156-3C11) Mouse mAb #3570 (solid line) compared to concentration-matched Mouse (E5Y6Q) mAb IgG2a Isotype Control (dashed line). Anti-mouse IgG (H+L) F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mouse embryonic fibroblasts treated with tunicamycin (2ug/ml, 10hr) and either Mouse (E5Y6Q) mAb IgG2a Isotype Control, Normal Rabbit IgG #2729, or CHOP (L63F7) Mouse mAb #2895 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.