GPX4 Inhibitor Screening Assay Kit

A convenient colorimetric method of screening human GPX4 inhibitors

Cayman's GPX4 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human GPX4, a negative regulator of the ferroptosis pathway. The assay measures GPX4 indirectly by a coupled reaction with glutathione reductase (GR). Oxidized glutathione (GSSG), produced upon reduction of hydroperoxide by GPX4, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in A340 is directly proportional to GPX4 activity. The GPX4 inhibitor ML-162 is included as a positive control.

配制试剂（磷酸盐缓冲液）、谷胱甘肽过氧化酶、烟酰胺腺嘌呤二核苷酸磷酸、谷胱甘肽、谷胱甘肽还原酶、异丙苯过氧化物溶液、二甲基亚砜溶液、半容积96孔板、96孔封板

Glutathione Peroxidase 4|PhGPx|Phospholipid Hydroperoxide Glutathione Peroxidase

≥ 2 years