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Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb

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Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb

品牌： CST

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反应种属：

Human,Mouse,Rat,Monkey,

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产品介绍

产品信息

抗原名称

GSK-3beta

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser9 of human GSK-3β.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying GSK3-beta (Ser9) phosphate. Cited in 578 publications. Validated for WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-GSK-3β (Ser9) (5B3) Rabbit Monoclonal Antibody (CST #9323) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:50
Immunofluorescence (Immunocytochemistry)	1:100

分子量

研究领域

癌症,纤维化,代谢,神经科学,

应用

反应种属

Human,Mouse,Rat,Monkey,

目标/特异性

Specificity/Sensitivity

Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb detects endogenous levels of GSK-3β only when phosphorylated at Ser9. The antibody may cross-react weakly with the phosphorylated form of GSK-3α due to high sequence homology.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). 1.Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9. 2.Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41. 3.Cross, D.A. et al. (1995) Nature 378, 785-9. 4.Nusse, R. (1997) Cell 89, 321-3. 5.Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

研究领域

癌症,纤维化,代谢,神经科学,

翻译后修饰

Phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #25462.

数据库链接

Entrez-Gene ID

2932

UniProt ID

P49841

参考图片

Western blot analysis of extracts from NIH/3T3 cells, λ-phosphatase- or PDGF-treated, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower).

Western blot 分析NIH/3T3 细胞， λ磷酸酶和PDGF处理组，所用抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗 (上) 或GSK-3β (27C10) Rabbit mAb兔单抗 #9315 (下)。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-GSK3β (Ser9) (5B3) Rabbit mAb.

免疫组化分析石蜡包埋的人乳腺癌细胞，所用抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗。

Immunohistochemical analysis of paraffin-embedded wild type (left) or GSK-3β (-/-) MEFs, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb. (MEF wild type, GSK-3α knock out and GSK-3β knock out cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).免疫组化分析石蜡包埋的野生型或者GSK-3β (-/-) MEFs细胞，所用抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb。)。（MEF 野生型, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞是由加拿大多伦多大学的Jim Woodgett博士友情提供。）

Immunohistochemical analysis using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb on SignalSlide(TM) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right)).

免疫组化分析SignalSlide(TM) Phospho-Akt (Ser473) IHC 对照#8101（分别为未处理（左）或者 LY294002处理的石蜡包埋的LNCaP细胞），使用的抗体是Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗。

Confocal immunofluorescent analysis of wild type (left), GSK-3α (-/-) (center), or GSK-3β (-/-) (right) mouse embryonic fibroblasts (MEFs), FGF-treated (top) or phosphatase-treated (bottom), using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

免疫共聚焦分析分析野生型（左），GSK-3α (-/-) (中) 与 GSK-3β (-/-) (右) 的小鼠胚胎成纤维细胞（MEFs），并分为FGF 处理（上）和phosphatase处理组（下），使用的抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (绿色)。肌动蛋白纤维被DY-554环肽所染色（红色）。Blue pseudocolor为 DRAQ5® #4084 （ DNA染料的荧光)。（MEF 野生型, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞由加拿大多伦多大学的Jim Woodgett博士友情提供。）

Western blot analysis of extracts from wild type (lanes 1,2), GSK-3α (-/-) (lanes 3,4) and GSK-3β (-/-) (lanes 5,6) mouse embryonic fibroblast cells (MEF), untreated or PDGF treated, using Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb (upper) and GSK-3α/β Antibody (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Western blot分析野生型（通道1和2），GSK-3α (-/-) (通道 3和4) 与 GSK-3β (-/-) (通道5和6) 的小鼠胚胎成纤维细胞，并分为未处理和PDGF处理组，使用的抗体为Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb兔单抗 (上) 和 GSK-3α/β Antibody (下)。（MEF 野生型, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞是由加拿大多伦多大学的Jim Woodgett博士友情提供。）

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货号：

9323T

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