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hGlut1 MAb (Cl 20291 (200 ug)
hGlut1 MAb (Cl 20291 (200 ug)
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hGlut1 MAb (Cl 20291 (200 ug)

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品牌： R&D Systems
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    来源宿主：
    Mouse IgG2b
    Mouse IgG2b
    实验应用：
    Flow Cytometry(2.5 µg/106 cells)
    Immunocytochemistry(8-25 µg/mL)
    Flow Cytometry(2.5 µg/106 cells)
    Immunocytochemistry(8-25 µg/mL)
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A or G purified from hybridoma culture supernatant
    抗原名称
    Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)， CSE， DYT17， DYT18， DYT9， EIG12， G
    宿主
    Mouse IgG2b
    免疫原
    NS0 mouse myeloma cell line transfected with human Glut1
    Met1-Val492
    Accession # AAA52571
    克隆号
    202915

    Scientific Data

    Flow Cytometry Detection of Glut1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.View Larger

    Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

    Flow Cytometry Detection of Glut1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.View Larger

    Detection of Glut1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

    Immunocytochemistry Glut1 antibody in HepG2 Human Hepatocellular Carcinoma Cell Line by Immunocytochemistry (ICC).View Larger

    Glut1 in HepG2 Human Hepatocellular Carcinoma Cell Line. Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

    Human Glut1 Antibody Summary

    Species Reactivity
    Human
    Specificity
    Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
    Source
    Monoclonal Mouse IgG2B Clone # 202915
    Purification
    Protein A or G purified from hybridoma culture supernatant
    Immunogen
    NS0 mouse myeloma cell line transfected with human Glut1
    Met1-Val492
    Accession # AAA52571
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Label
    Unconjugated

    Applications

    Recommended Concentration
    Sample
    Flow Cytometry
    0.25 µg/106 cells
    See below
    CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
     
    Immunocytochemistry
    8-25 µg/mL
    See below

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Flow Cytometry(2.5 µg/106 cells)
    Immunocytochemistry(8-25 µg/mL)
    背景
    别名
    Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis),CSE,DYT17,DYT18,DYT9,EIG12,Glucose transporter type 1, erythrocyte/brain,Glut1,GLUT-1,GLUT1DS,HepG2 glucose transporter,HTLVR,Human T-Cell Leukemia Virus (I and II) Receptor,MGC141895,MGC141896,PED,SLC2A1,solute carrier family 2 (facilitated glucose transporter), member 1,Solute Carrier Family 2 Member 1,solute carrier family 2, facilitated glucose transporter member 1,Glucose Transporter Type 1
    背景

    Background: Glut1

    Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

    References
    1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
    2. Meuckler, M. et al. 1985, Science 229:941.
    3. Jones, K.S. et al. 2006, J. Virol. 8291.
    4. Takenouchi, N. et al. 2007, J. Virol. 1506.
    5. Kinet, S. et al. 2007, Retrovirology 4:31.
    Long Name
    Glucose Transporter Type 1
    Entrez Gene IDs
    6513 (Human); 20525 (Mouse)
    Alternate Names
    Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis); CSE; DYT17; DYT18; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; Glut1; GLUT-1; GLUT1DS; HepG2 glucose transporter; HTLVR; Human T-Cell Leukemia Virus (I and II) Receptor; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; Solute Carrier Family 2 Member 1; solute carrier family 2, facilitated glucose transporter member 1
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Loading...
    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    参考图片

    Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

    Detection of Glut1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

    Glut1 in HepG2 Human Hepatocellular Carcinoma Cell Line. Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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    货号：
    MAB1418
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    询价
    25ug
    200ug
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