首页抗体一抗一抗

hGlut1 MAb (Cl 20292 (25 ug)

1/2

hGlut1 MAb (Cl 20292 (25 ug)

品牌： R&D Systems

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

来源宿主：

Mouse IgG3

实验应用：

Western Blot(2 µg/mL)
Simple Western(20 µg/mL)

产品介绍

产品信息

耦联标记

Unconjugated

纯化方式

Protein A or G purified from hybridoma culture supernatant

抗原名称

Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)， CSE， DYT17， DYT18， DYT9， EIG12， G

宿主

Mouse IgG3

免疫原

NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571

克隆号

202921

Scientific Data

Western Blot

View Larger

Detection of Human Glut1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human Glut1 by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glut1 at approximately 69 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Human Glut1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glut1 in ELISAs.

Source

Monoclonal Mouse IgG₃ Clone # 202921

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用

实验应用

Western Blot(2 µg/mL)
Simple Western(20 µg/mL)

背景

别名

Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis),CSE,DYT17,DYT18,DYT9,EIG12,Glucose transporter type 1, erythrocyte/brain,Glut1,GLUT-1,GLUT1DS,HepG2 glucose transporter,HTLVR,Human T-Cell Leukemia Virus (I and II) Receptor,MGC141895,MGC141896,PED,SLC2A1,solute carrier family 2 (facilitated glucose transporter), member 1,Solute Carrier Family 2 Member 1,solute carrier family 2, facilitated glucose transporter member 1,Glucose Transporter Type 1

背景

Background: Glut1

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8⁺ T cells, and erythrocytes (1‑5). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

References

Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
Meuckler, M. et al. 1985, Science 229:941.
Jones, K.S. et al. 2006, J. Virol. 8291.
Takenouchi, N. et al. 2007, J. Virol. 1506.
Kinet, S. et al. 2007, Retrovirology 4:31.

Long Name

Glucose Transporter Type 1

Entrez Gene IDs

6513 (Human); 20525 (Mouse)

Alternate Names

Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis); CSE; DYT17; DYT18; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; Glut1; GLUT-1; GLUT1DS; HepG2 glucose transporter; HTLVR; Human T-Cell Leukemia Virus (I and II) Receptor; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; Solute Carrier Family 2 Member 1; solute carrier family 2, facilitated glucose transporter member 1

研究领域

Related Research Areas

制备和贮存

溶解方法

Reconstitute at 0.5 mg/mL in sterile PBS.

保存方式

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Detection of Human Glut1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Glut1 by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glut1 at approximately 69 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

MAB14181-SP

一键复制

询价

25ug

100ug

选择数量

当前规格1件起购

配送至

预计2-3周送达，快递: 免运费，若需干冰额外收费

询价大包装询价