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Mono-Methyl-Histone H3 (Lys4) (D1A9) XP ® Rabbit mAb
Mono-Methyl-Histone H3 (Lys4) (D1A9) XP ® Rabbit mAb

Mono-Methyl-Histone H3 (Lys4) (D1A9) XP ® Rabbit mAb

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品牌： CST
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    分子量：
    17
    17
    反应种属：
    Human,Mouse,Rat,Monkey,
    Human,Mouse,Rat,Monkey,
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    产品介绍
    产品信息
    抗原名称
    Histone H3
    来源纯化
    使用与组蛋白 H3（赖氨酸在此 4 被单甲基化）氨基末端相对应的合成肽，对动物进行免疫接种来产生单克隆抗体。
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying H3 (Lys4) methylate. Cited in 128 publications. Validated for WB, IF, F, ChIP, ChIP, C&R, C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit Monoclonal Antibody (CST #5326) is ready to ship.
    商品描述

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652. The CUT&Tag dilution was determined using 100,000 cells in a total reaction volume of 100 μl.

    ApplicationDilution
    Western Blotting1:1000
    Immunofluorescence (Immunocytochemistry)1:200 - 1:800
    Flow Cytometry (Fixed/Permeabilized)1:200 - 1:800
    Chromatin IP1:50
    Chromatin IP-seq1:50
    CUT&RUN1:50
    CUT&Tag1:50
    分子量
    17
    内毒素水平
    黑腹果蝇
    研究领域
    癌症,发育生物学与干细胞研究,表观遗传学,神经科学,
    应用
    反应种属
    Human,Mouse,Rat,Monkey,
    预测反应种属
    D.melanogaster,
    目标/特异性

    Specificity/Sensitivity

    Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb 可检测仅在 Lys4 位点被单甲基化的组蛋白 H3 的内源水平。该抗体不与非甲基化、二甲基化或三甲基化的组蛋白 H3 Lys4 发生交叉反应。此外，抗体不与甲基化组蛋白 H3 Lys9、Lys27、Lys36 或甲基化组蛋白 H4 Lys20 发生交叉反应。

    物种反应性：

    人, 小鼠, 大鼠, 猴

    敏感性
    Endogenous
    背景
    背景
    核小体由四个核心组蛋白（H2A、H2B、H3 和 H4）组成，是染色质的主要组成部分。最初被认为可作为 DNA 包装的静态支架的组蛋白现在已被证明是动态蛋白，可进行多种类型的翻译后修饰，包括乙酰化、磷酸化、甲基化和泛素化 (1)。组蛋白甲基化是形成基因组的活跃和非活跃区域的主要决定因素，并且在发育期间基因组的正确编程过程中发挥关键作用 (2,3)。组蛋白 H3 (Arg2, 17, 26) 和 H4 (Arg3) 的精氨酸甲基化可促进转录激活，并由蛋白精氨酸甲基转移酶 (PRMTs) 家族介导，该家族包括辅助激活因子 PRMT1 和 CARM1 (PRMT4) (4)。与此相反，已确定更多样化的组蛋白赖氨酸甲基转移酶，除了其中某个外其余的都含有一个保守催化 SET 结构域，该结构域最初在果蝇 Su(var)3-9、zeste 增强子和 Trithorax 蛋白中得以确认。赖氨酸甲基化主要发生在组蛋白 H3 (Lys4 位点, 9, 27, 36, 79) 和 H4 (Lys20 位点) 中，并已确定与转录激活和沉默有关 (4)。这些赖氨酸残基的甲基化可协调染色质修饰酶的募集，这些酶含有甲基赖氨酸结合模块，例如克罗莫结构域 (HP1, PRC1)、PHD fingers (BPTF, ING2)、tudor 结构域 (53BP1) 和 WD-40 结构域 (WDR5) (5-8)。PADI4、LSD1、JMJD1、JMJD2 和 JHDM1 等组蛋白脱甲基酶的发现表明：甲基化是可逆的表观遗传标记物 (9)。 1.Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51. 2.Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop, 1-27. 3.Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42. 4.Lee, D.Y. et al. (2005) Endocr Rev 26, 147-70. 5.Daniel, J.A. et al. (2005) Cell Cycle 4, 919-26. 6.Shi, X. et al. (2006) Nature 442, 96-9. 7.Wysocka, J. et al. (2006) Nature 442, 86-90. 8.Wysocka, J. et al. (2005) Cell 121, 859-72. 9.Trojer, P. and Reinberg, D. (2006) Cell 125, 213-7.
    研究领域
    癌症,发育生物学与干细胞研究,表观遗传学,神经科学,
    翻译后修饰
    Methylate
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
    数据库链接
    Entrez-Gene ID
    8350
    UniProt ID
    P68431

    参考图片

    Confocal immunofluorescent analysis of HeLa cells using Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (blue). Actin filaments were labeled with Dy-554 phalloidin (red).

    使用Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb 兔单抗(绿色)和MEK1/2 (L38C12) Mouse mAb #4694 鼠单抗(蓝色)，共聚焦免疫荧光分析HeLa细胞。Dy-554 phalloidin标记微丝蛋白(红色)。

    Western blot analysis of extracts from various cell lines using Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb.

    使用Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb兔单抗，免疫印迹(Western blot)分析不同细胞中Mono-Methyl-Histone H3 (Lys4)的蛋白水平。

    Mono-Methyl Histone H3 (Lys4) (D1A9) XP® Rabbit mAb specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated mono-methyl histone H3 (Lys4) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the mono-methyl histone H3 (Lys4) peptide competed away binding of the antibody.

    通过peptide ELISA确定Mono-Methyl Histone H3 (Lys4) (D1A9) XP® Rabbit mAb兔单抗的特异性。该图描述了抗体与提前包被的mono-methyl histone H3 (Lys4) peptide的结合能力，并且多肽中含有浓度递增的不同竞争多肽。如同所示，仅mono-methyl histone H3 (Lys4) peptide竞争脱离抗体的结合。

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 K562 cells and either 10 μl of Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ADAM9 Intron 11 Primers #73401, human ADAM18 intron 14 primers, human Trio inton 1 primers, and SimpleChIP® Human Trio Exon 57 Primers #90568. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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    货号：
    5326T
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