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HA-Tag (C29F4) Rabbit mAb
HA-Tag (C29F4) Rabbit mAb
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HA-Tag (C29F4) Rabbit mAb

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品牌： CST
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    反应种属：
    All
    All
    来源宿主：
    Rabbit
    Rabbit
    产品介绍
    产品信息
    抗原名称
    HA-Tag
    来源纯化
    通过使用含有流感血凝素表位 (YPYDVPDYA) 的合成肽对动物进行免疫接种来产生单克隆抗体。
    宿主
    Rabbit
    简单描述
    Discover our highly specific anti HA antibody for precise detection and analysis. Click here to learn more about its applications and benefits.
    商品描述

    Product Usage Information

    For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    ApplicationDilution
    Western Blotting1:1000
    Simple Western™1:50 - 1:250
    Immunoprecipitation1:50
    Immunohistochemistry (Paraffin)1:800 - 1:3200
    Immunofluorescence (Immunocytochemistry)1:800 - 1:1600
    Flow Cytometry (Fixed/Permeabilized)1:800 - 1:1600
    Chromatin IP1:50
    应用
    反应种属
    All
    目标/特异性

    Specificity/Sensitivity

    HA-Tag (C29F4) Rabbit mAb 可检测含有 HA 表位标签的外源表达蛋白质。该抗体可与未知来源的 ~100kDa 蛋白发生交叉反应。

    物种反应性：

    预期的所有物种

    敏感性
    Transfected Only
    背景
    背景
    在使用免疫印迹法、免疫沉淀法和免疫染色技术对蛋白进行标记和检测时，表位标签十分有用。由于分子量小，它们不太可能对标记蛋白的生化特性造成影响。HA 标签衍生自流感血凝素蛋白的表位，该表位已经广泛用作表达载体中的通用表位标签 (1)。 1.Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #86124.

    参考图片

    Flow cytometric analysis of HeLa cells, untransfected (blue) or transfected with HA-FoxO4 (green), using HA-Tag (C29F4) Rabbit mAb #3724.流式细胞术分析HeLa细胞，蓝色为未转染组，绿色为HA-FoxO4 转染组，所用抗体为HA-Tag (C29F4) Rabbit mAb #3724。

    Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (right) or HA-Tag transfected (left), using HA-Tag (C29F4) Rabbit mAb.免疫组化分析石蜡包埋的COS细胞，下图为未转染组，上图为HA-Tag转染组，所用抗体为HA-Tag (C29F4) Rabbit mAb。

    Western blot analysis of extracts from HeLa cells, untransfected or transfected with either HA-FoxO4 or HA-Akt3, using HA-Tag (C29F4) Rabbit mAb.Weatern blot分析HeLa细胞提取物，未转染组和采用 HA-FoxO4 或 HA-Akt3转染组,所用抗体为HA-Tag (C29F4) Rabbit mAb。

    Confocal immunofluorescent analysis of COS cells, transfected with an HA-tagged protein (left) or mock-transfected (right), using HA-Tag (C29F4) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).免疫荧光分析COS-7细胞提取物。左图为采用HA标签蛋白转染组，下图为mock转染组，所用抗体为HA-Tag (C29F4) Rabbit （绿色），肌动蛋白丝采用DY-554 phalloidin (红色)标记。蓝色伪彩为荧光DNA染料，信息为DRAQ5 ® #4084 。

    293T cells were either untransfected (left panel) or transfected with an HA-tagged human CBP construct (right panel), then treated with Forskolin #3828 (30 µM) . Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 10 µl of HA-Tag (C29F4) Rabbit mAb, 10 µl of CBP (D9B6) Rabbit mAb, or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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    货号：
    3724T
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