Mouse Hematopoietic Progenitor (Stem) Cell Enrichment Set - DM

Magnetic Bead

The BD IMag™ Mouse Hematopoietic Progenitor Cell Enrichment Set - DM reacts with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, and erythrocytes. The BD IMag™ Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. This Set is designed for the immunomagnetic enrichment of hematopoietic progenitors from mouse bone marrow by depletion of cells committed to the T- and B-lymphocytic, myeloid (monocytic and granulocytic), and erythroid lineages. The Set contains sufficient reagents to label 10^9 bone marrow cells.   Biotin Mouse Lineage Depletion Cocktail is comprised of the following biotin-conjugated monoclonal antibodies: Anti-mouse CD3e, clone 145-2C11                                              Anti-mouse CD11, clone M1/70 Anti-mouse CD45R/B220, clone RA3-6B2                                     Anti-mouse Ly-6G and Ly-6C (Gr-1), clone RB6-8C5 Anti-mouse TER-119/Erythroid Cells, clone TER-119

Cell separation (Routinely Tested)

Mouse (QC Testing)

研发参考(6) 1. Goodell MA, Rosenzweig M, Kim H, et al. Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species. Nat Med. 1997; 3(12):1337-1345. (Biology). 2. Morrison SJ, Wandycz AM, Hemmati HD, Wright DE, Weissman IL. Identification of a lineage of multipotent hematopoietic progenitors. Development. 1997; 124(10):1929-1939. (Biology). 3. Osawa M, Tokumoto Y, Nakauchi H. Hematopoietic stem cells. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology, 5th Edition. Cambridge: Blackwell Science; 1996:66.1-66.5. 4. Sato T, Laver JH, Ogawa M. Reversible expression of CD34 by murine hematopoietic stem cells. Blood. 1999; 94(8):2548-2554. (Biology). 5. Spangrude GJ, Heimfeld S, Weissman IL. Purification and characterization of mouse hematopoietic stem cells. Science. 1988; 241(4861):58-62. (Biology). 6. Spangrude GJ, Scollay R. A simplified method for enrichment of mouse hematopoietic stem cells. Exp Hematol. 1990; 18(8):920-926. (Biology).