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rhHGF, CF (5 ug)
rhHGF, CF (5 ug)
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rhHGF, CF (5 ug)

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    Scientific Data

    Bioactivity Recombinant Human HGF Protein BioactivityView Larger

    Recombinant Human HGF (294-HG) induces IL-11 secretion in Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to less than 4.00 ng/mL.

    Cell Culture View Larger

    Adult stem cell-derived liver organoids were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and liver organoid initiation medium, which includes Recombinant Human HGF (Catalog # 294-HG), Recombinant Human BMP-7 (354-BP), Recombinant Human EGF (236-EG), Recombinant Human FGF-10 (345-FG), Recombinant Human Noggin (6057-NG), Recombinant Human R-Spondin 1 (4645-RS), and Recombinant Human Wnt-3a (5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human HGF (Catalog # 294-HG), Recombinant Human BMP-7 (354-BP), Recombinant Human EGF (236-EG), and Recombinant Human FGF-19 (969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (AF2400; red) and counterstained with DAPI (5748; blue).

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    294-HG

    FormulationLyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    294-HG/CF

    FormulationLyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
    ReconstitutionReconstitute at 100 µg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Human HGF Protein Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is equal to or less than 4.00 ng/mL.

    Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HGF protein
    Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
    Accession #
    P14210.2
    N-terminal Sequence
    Analysis
    alpha chain: No results obtained; Gln32 predicted
    beta chain: Val495
    Structure / Form
    Disulfide-linked heterodimer
    Predicted Molecular Mass
    53.7 kDa ( alpha chain), 26 kDa ( beta chain)
    SDS-PAGE
    60-70 kDa, non-reducing conditions
    60 kDa, and 33-34 kDa, reducing conditions
    背景
    背景

    Background: HGF

    HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
    disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates human HGF isoforms that lack the proteinase‑like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH‑stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

    References
    1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
    2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
    3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
    4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
    5. Nakamura, T. et al. (1989) Nature 342:440.
    6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
    7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
    8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
    9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
    10. Montesano, R. et al. (1991) Cell 67:901.
    11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
    12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
    13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
    14. Mineo, R. et al. (1994) Endocrinology 145:4355.
    15. Urbanek, K. et al. (2005) Circ. Res. 97:663.
    Long Name
    Hepatocyte Growth Factor
    Entrez Gene IDs
    3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)
    Alternate Names
    deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
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    货号：
    294-HG-005/CF
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