HTRF HUMAN IFN ALPHA KIT - 500 PTS

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HTRF HUMAN IFN ALPHA KIT - 500 PTS

品牌： Revvity

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产品介绍

产品信息

Overview

Mainly produced by leukocytes, IFN alpha is involved in innate immunity response to viral infection. IFN alpha activates several intracellular pathways in target cells, which explains its pleiotropic effects on cell differentiation, growth, and immunoregulation.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

Specifications

Other Specifications

Assay Target Type	Kit
Unit Size	100,000 Assay Points

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Assay details

Technical specifications of human IFN alpha kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	11 pg/mL &36 pg/mL
Calibration	NIBSC (94/784) value (IU/mL) = 0,9 x HTRF hIFNa HTRF
Range	36 4,000 pg/mL
Time to result	2h at RT
Species	Human only

Analytical performance

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [IFNa] (pg/mL)	CV
1	150	11%
2	1500	2%
3	3000	5%
	Mean CV	6%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[IFNa] (pg/mL)	Mean (delta R)	CV
1	47	268	9%
2	206	1150	5%
3	1905	4227	4%
		Mean CV	6%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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货号：

62HIFNAPEG

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