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HTRF IP-ONE GQ KIT - 1K PTS
HTRF IP-ONE GQ KIT - 1K PTS

HTRF IP-ONE GQ KIT - 1K PTS

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    产品介绍
    产品信息

    Overview

    HTRF IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.

    From high-throughput screening (HTS) to structure-activity relationships (SAR), IP-One has been shown to offer superior benefits over traditional assays

    Specifications

    Assay Target Type
    Kit
    Brand
    HTRF
    Therapeutic Area
    Cardiovascular
    Infectious Diseases
    Metabolism/Diabetes
    NASH/Fibrosis
    Neuroscience
    Oncology & Inflammation
    Rare Diseases
    Unit Size
    100,000 Assay Points

    How it works

    Assay principle

    The kit is based on a competitive format involving a specific antibody labeled with cryptate (donor) and IP1 coupled to d2 (acceptor). Native IP1 produced by cells competes with d2-labeled IP1 for binding to monoclonal anti-IP1 Tb2+ cryptate. LiCl is added to the cell stimulation buffer, causing IP1 to accumulate upon receptor activation.

     

    1gpcr-how-it-works-assay-principle-ip-one-62ipapeb-62ipapec-62ipapej.svg

     

    Assay protocol

    The IP-One assay features a streamlined protocol with just two incubation steps: Cell stimulation by the ligand or target compounds IP1 detection using HTRF reagentsThis protocol requires only a single, one-hour incubation period following cell stimulation.

     

    2gpcr-how-it-works-assay-protocol-ip-one-62ipapeb-62ipapec-62ipapej.svg

     

    Assay details

    Specificity of IP-One Gq assay

    IP-One ensures signal specificity, measuring only IP-1, an analyte that is solely produced by the action of the phospholipase enzyme (PLC) and mediated by GPCRs. 

    In contrast, calcium responses are complex in nature. Calcium release is frequently triggered by non-G protein mechanisms, including Ca2+ permeable channels, calcium pumps, calcium transporters.

     

    Standard curve characteristics

    Assay Window, sensitivity, reproducibility and specificity : IP-One makes no concession !

     

    4specifications-ip-one-gq-1.svg

     

    参考图片

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    货号：
    62IPAPEB
    一键复制
    询价
    1000Tests
    20000Tests
    100000Tests
    选择数量
    当前规格1件起购 
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