HTRF MOUSE IFN G KIT - 500 PTS

品牌： Revvity

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产品介绍

产品信息

Overview

IFN gamma is a pro-inflammatory cytokine secreted by T and NK cells in response to viral infection and or inflammatory conditions. IFN gamma plays a major role in innate and adaptive immunity by promoting T cell differentiation into TH1 and development of Treg, but also by inhibiting T cell differentiation into TH17. Its anti-tumor properties have been used in treatment of osteoporosis and it is of great interest in immuno-oncology, where it improves survival in patients with bladder or ovarian carcinoma.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.

Specifications

Other Specifications

Assay Target Type	Kit
Brand	HTRF
Therapeutic Area	Oncology & Inflammation
Unit Size	100,000 Assay Points

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting,

Revvity has also worked with Myassays.com to help you in your data analysis, you’ll be able to access a free online software to run your IFN gamma analysis.

Assay details

Technical specifications of mouse IFN gamma kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	23 pg/mL &34 pg/mL
Range	34 3,000 pg/mL
Time to result	2h at RT
Species	Mouse only

Analytical performance

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [IFNg] (pg/mL)	CV
1	80	11%
2	1375	2%
3	2750	2%
	Mean CV	5%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[IFNg] (pg/mL)	Mean (delta R)	CV
1	94	213	5%
2	375	868	10%
3	1500	3502	13%
		Mean CV	9%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

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货号：

62MIFNGPEG

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