BD™ ELISPOT Human IFN-γ ELISPOT Pair

IFN-γ

The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro.  Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis. This product contains sufficient reagents for five 96-well plates, including unlabelled anti-cytokine capture antibody (no azide/low endotoxin format);  biotinylated anti-cytokine detection antibody and a Certificate of Analysis that provides lot-specific optimal reagent concentrations.

The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro.  Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis. This product contains sufficient reagents for five 96-well plates, including unlabelled anti-cytokine capture antibody (no azide/low endotoxin format);  biotinylated anti-cytokine detection antibody and a Certificate of Analysis that provides lot-specific optimal reagent concentrations.

(RUO)

IFN-γ

研发参考(5) 1. Czerkinsky C, Andersson G, Ekre HP, Nilsson LA, Klareskog L, Ouchterlony O. Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells. J Immunol Methods. 1988; 110(1):29-36. (Methodology). 2. Fujihashi K, McGhee JR, Beagley KW, et al. Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells. J Immunol Methods. 1993; 160(2):181-189. (Methodology). 3. Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M. Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients. J Immunol. 2000; 164(7):3723-3732. (Methodology). 4. Ronnblom L, Cederblad B, Sandberg K, Alm GV. Determination of herpes simplex virus-induced alpha interferon-secreting human blood leucocytes by a filter immuno-plaque assay. Scand J Immunol. 1988; 27(2):165-170. (Methodology). 5. Sedgwick JD, Holt PG. A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J Immunol Methods. 1983; 57(1-3):301-309. (Methodology).